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The Biochemical journal

The isolation of human-erythrocyte band-3 polypeptide labelled with a photosensitive hydrophobic probe.


PMID 7188423

Abstract

To investigate the intramembranous domains of the major band-3 polypeptide, human erythrocyte membranes were labelled with 1-azido-4-[125I]iodobenzene. The anion-exchange protein has been isolated by a new procedure that decreases possible contamination by other integral membrane proteins of similar molecular weight. After chemical modification, the protein was subjected to proteolytic cleavage by several proteinases to yield a distinct pattern of peptides, a few of which contained radioactivity.

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779482
1-Azido-4-iodobenzene solution, 0.5 M in tert-butyl methyl ether, ≥95% (HPLC)
C6H4IN3