EMAIL THIS PAGE TO A FRIEND

Journal of chromatography

Methods for recovering nucleic acid fragments from agarose gels.


PMID 7693742

Abstract

Agarose gel electrophoresis is a powerful technique for the separation of nucleic acids on the basis of their size and conformation. The development of methods to recover size-fractionated nucleic acids molecules from agarose gels has greatly facilitated recombinant DNA technologies. Although several methods for recovering DNA and RNA molecules have been developed during the past fifteen years, none of them has been universally accepted. In this review we describe, discuss and evaluate the most common procedures with which we have had experience. Our evaluation is based on the criteria of yield, purity, speed, simplicity and low cost. We have considered three different approaches to the problem of recovering nucleic acids: chemical gel dissolution, physical gel disruption and physical extrusion from intact gels.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

A6013
Agarose, Type I, low EEO
A6877
Agarose, Medium EEO, for molecular biology
A0169
Agarose, Type I-A, low EEO
A4718
Agarose, High resolution, for molecular biology
A4679
Agarose, Low EEO, for Immunoelectrophoresis
A7431
Agarose, BioReagent, for molecular biology, Wide range/Standard 3:1
A9918
Agarose, Type II-A, Medium EEO
A0576
Agarose, Low EEO
A2790
Agarose, Wide range, for molecular biology
A2929
Agarose, For pulsed field electrophoresis running gel
A3038
Agarose, Type XI, low gelling temperature, suitable for separation of small nucleic acid fragments
A5030
Agarose, Ultra-low Gelling Temperature
A3643
Agarose, Type IV, Special High EEO
A2576
Agarose, Ultra-low Gelling Temperature, molecular biology grade
A9793
Agarose, High EEO
A7174
Agarose, High Gelling Temperature
A5304
Agarose, Special High EEO
A3054
Agarose, For pulsed field electrophoresis sample preparation
05066
Agarose, High EEO, for molecular biology
4B200
Sepharose® 4B, 45-165 μm bead diameter
GE17-0120-01
Sepharose® 4B, GE Healthcare, 17-0120-01, pack of 1 L
6B100
Sepharose® 6B, 6% Beaded Agarose, 45-165 μm (wet), fractionation range 10,000-1,000,000 Da (dextrans)
GE17-0110-01
Sepharose® 6B, GE Healthcare, 17-0110-01, pack of 1 L
U0507
Ultrogel® A4, aqueous ethanol suspension, 60-140 μm (wet bead diameter), exclusion limit 20,000,000 Da, fractionation range 55,000-9,000,000 Da (globular proteins)