EMAIL THIS PAGE TO A FRIEND

Chinese medical sciences journal = Chung-kuo i hsueh k'o hsueh tsa chih

The autocrine regulatory effect of vasoactive intestinal peptide on the growth of human pancreatic carcinoma cells.


PMID 7718859

Abstract

In the present study, the effects of VIP on the growth of two human pancreatic carcinoma cell lines PU-PAN-1 and PANC-1 were determined using tritiated thymidine incorporation. VIP receptors, intracellular cAMP and polyamines were investigated. The results indicated that VIP at a concentration of 10(-8) mol/L to 10(-7) mol/L can significantly stimulate the growth of PU-PAN-1 cells but not PANC-1 cells. This effect is dose-dependent and abolished by VIP receptor antagonist, [4-C1-Phe6, Leu17] VIP, suggesting VIP receptors in PU-PAN-1 cells may mediate this effect. VIP can markedly elevate the levels of intracellular cAMP and polyamines in PU-PAN-1 cells, indicating that the growth-promoting effect stimulated by VIP may be via a rapid increase in the biosyntheses of cAMP and polyamines. In addition, the VIP-antibody inhibited the growth of PU-PAN-1 cells in serum-free culture medium. The results above suggested that VIP has an autocrine regulatory effect on this pancreatic carcinoma cell line (PU-PAN-1).

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

V4380
[D-p-Cl-Phe6, Leu17]-Vasoactive Intestinal Peptide human, porcine, rat, ≥97% (HPLC)
C148H239ClN44O42