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Journal of biochemical and biophysical methods

Determination of singlet oxygen quantum yields with 1,3-diphenylisobenzofuran in model membrane systems.


PMID 8227944

Abstract

The oxidation of 1,3-diphenylisobenzofuran by singlet oxygen was investigated in methanol and in two different types of liposomes. It was found that at high concentrations of scavenger 1,3-diphenylisobenzofuran, e.g., > 100 microM in methanol, the 1:1 oxidation stoichiometry is lost and more than one scavenger molecule per molecule of singlet oxygen is consumed. In model membrane systems, where local scavenger concentrations are high due to compartmentalization, correct singlet oxygen quantum yields with 1,3-diphenylisobenzofuran are only determined if the increased oxidation is taken into account.

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105481
1,3-Diphenylisobenzofuran, 97%
C20H14O