EMAIL THIS PAGE TO A FRIEND

Journal of chromatography. B, Biomedical sciences and applications

Simple approach for analysis of plasma oxo-, hydroxy- and dicarboxylic acids.


PMID 9210459

Abstract

Following deproteinization of plasma with organic solvents the supernatant was shaken with hexane and cation-exchange resin in an Eppendorf tube to remove fatty and amino acids and the medium was subjected to direct treatment with ethyl chloroformate under catalytic influence of pyridine. A subsequent extraction of the immediately formed ethyl esters with a drop of chloroform enabled us to subject the sample to gas chromatographic (GC) analysis. Since ketocarboxylic acids do not require a preliminary oximation the total time of sample workup and analysis takes only several minutes.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

185892
Ethyl chloroformate, 97%
C3H5ClO2