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A method for the measurement of ultra-low amounts of prostaglandins in inflammatory exudates using gas chromatography and mass spectrometry.


PMID 941791

Abstract

A GC-MS assay for PGE2 and PGF2alpha has been described. The method is highly specific and sensitive down to the sub-picogram level. The assay has been used to measure the time dependence of PG concentration in carrageenan-induced inflammatory exudates and the results compared with a bioassay. Estimates of the extraction and purification yield of PGs from exudates have also been obtained.

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