EMAIL THIS PAGE TO A FRIEND

Applied and environmental microbiology

Green fluorescent protein as a noninvasive stress probe in resting Escherichia coli cells.


PMID 9925561

Abstract

We constructed and characterized three stress probe plasmids which utilize a green fluorescent protein as a noninvasive reporter in order to elucidate Escherichia coli cellular stress responses in quiescent or resting cells. Cellular stress levels were easily detected by fusing three heat shock stress protein promoter elements, those of the heat shock transcription factor sigma32, the protease subunit ClpB, and the chaperone DnaK, to the reporter gene gfpuv. When perturbed by a chemical or physical stress (such as a heat shock, nutrient [amino acid] limitation, or addition of IPTG [isopropyl-beta-D-thiogalactopyranoside], acetic acid, ethanol, phenol, antifoam, or salt [osmotic shock]), the E. coli cells produced GFPuv, which was easily detected within the cells as emitted green fluorescence. Temporal and amplitudinal mapping of the responses was performed, and the results revealed regions where quantitative delineation of cell stress was afforded.

Related Materials

Product #

Image

Description

Molecular Formula

Add to Cart

S4503
DL-Serine hydroxamate, seryl-tRNA synthetase inhibitor
C3H8N2O3