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860336 Sigma-Aldrich

3,3′,5,5′-Tetramethylbenzidine

≥99%

Synonym: BM blue, Sure Blue TMB, TMB Blotting Plus, TMB substrate, TMB

  • CAS Number 54827-17-7

  • Linear Formula [-C6H2(CH3)2-4-NH2]2

  • Molecular Weight 240.34

  •  Beilstein/REAXYS Number 2808541

  •  EC Number 259-364-6

  •  MDL number MFCD00007748

  •  PubChem Substance ID 24888505

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Properties

Related Categories Amines, Building Blocks, C15 to C19, Chemical Synthesis, Nitrogen Compounds - Organic Building Blocks,
Quality Level   200
assay   ≥99%
mp   168-171 °C (lit.)
storage temp.   2-8°C
SMILES string   Cc1cc(cc(C)c1N)-c2cc(C)c(N)c(C)c2
InChI   1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3
InChI key   UAIUNKRWKOVEES-UHFFFAOYSA-N

Description

General description

3,3′,5,5′-Tetramethylbenzidine is a peroxidase sensitive dye. It is a better alternative to syringaldazine for the determination of chlorine in water due to its product stability and no interference from calcium and magnesium ions.

Packaging

1, 5, 25 g in glass bottle

100 mg in glass bottle

Application

3,3′,5,5′-Tetramethylbenzidine (TMB) is a noncarcinogenic substitute (Ames test negative) for benzidine suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm. A sensitive and specific reagent for the detection of blood, assay of hemoglobin, assay of peroxidases.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
RTECS 
DV2300000
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

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Peer-Reviewed Papers
15

References

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