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900751 Sigma-Aldrich

Biotinylated-D-lysine TFA salt

Synonym: (R)-2-Ammonio-6-(5-((3aR,4R,6aS)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamido)hexanoate 2,2,2-trifluoroacetate, BDL TFA salt

  • Empirical Formula (Hill Notation) C16H28N4O4S · C2HF3O2

  • Molecular Weight 486.51

  •  NACRES NA.22

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Properties

Related Categories Amino Acids & Derivatives, Chemical Biology, Chemical Synthesis, Lysine, Other Biological Tools,
Quality Level   100
form   powder or crystals
application(s)   peptide synthesis: suitable
storage temp.   −20°C
SMILES string   O=C(O)C(F)(F)F.[O-]C([C@@H](CCCCNC(CCCC[C@@H]1[C@@](NC(N2)=O)([H])[C@@]2([H])CS1)=O)[NH3+])=O

Description

Application

Biotinylated D-lysine (BDL) can be used to conjugate a biotin handle into bacterial peptidoglycan (PG) for various applications. For example, Suzanne Walker and coworkers reported that penicillin-binding protein PBP4 of Staphylococcus aureus could use BDL to biotinylate Lipid I and Lipid II, facilitating assays to measure infamously low-abundance pools of cellular lipid-linked PG precursors. BDL provides a tool for studying other aspects of PG assembly including antibacterial development efforts.

Packaging

10 mg in glass bottle

Other Notes

Detection of Lipid-Linked Peptidoglycan Precursors by Exploiting an Unexpected Transpeptidase Reaction

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles
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