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1.52007 Supelco

Chromolith® FastGradient RP-18 endcapped 50-2 HPLC column

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Properties

Related Categories Analytical/Chromatography, HPLC/UHPLC More...
Quality Level   100
feature   endcapped
parameter   200 bar pressure
  45 °C max. temp.
application(s)   UHPLC: suitable
  mass spectroscopy (MS): suitable (High)
matrix   highly porous
  monolithic
pore size   1.5 μm macroporosity
  13 nm mesoporosity
operating pH range   2 - 7.5
storage conditions   no restrictions.

Description

General description

All Chromolith® columns are made of monolithic silica with a characteristic bimodal pore structure. Chromolith 2mm columns have macropores with 1.6 µm diameter, giving a column efficiency exceeding 100,000 plates/meter. The mesopores are 13 nm (130 Å), the surface modification is octadecylsilan with full end-capping.

Analysis Note

Appearance: passes test
Pressure: ≤ 30 bar
Performance (anthracene)
Theoretical Plates (N/m) (Anthracene): ≥ 100000
Capacity factor (Anthracene): 3.6 - 4.3
Tailing (USP): 0.86 - 1.30 %
Selectivity
separation factor (anthracene/progesterone): 2.30 - 2.60

Legal Information

Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Chromolith® HPLC and UHPLC columns

Chromolith® HPLC and UHPLC columns are made from monolithic silica with a bimodal pore structure using sol-gel technology. There are no silica particles.
Keywords: High performance liquid chromatography, Sample preparations, Separation, Sol-gel

HPLC Analysis of Artificial Food Colorants Cochineal Red A and Azorubine in Yoghurt on Chromolith® FastGradient RP-18 endcapped

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Artificial Food Colorants Cochineal Red A and Azorubine in Yoghurt on Chromolith® FastGradient RP-18 endcapped
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Lapatinib on Chromolith® Performance 50-2mm

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Lapatinib on Chromolith® Performance 50-2mm
Keywords: High performance liquid chromatography, Pharmaceutical, Sample preparations

LC/MS Analysis of Annato (or Roucou or Achiote) on Chromolith® FastGradient RP-18 endcapped

From our library of Articles, Sigma-Aldrich presents LC/MS Analysis of Annato (or Roucou or Achiote) on Chromolith® FastGradient RP-18 endcapped
Keywords: Chromatography, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, transformation

LC/MS Analysis of Artificial Food Colorants Cochineal Red A and Azorubine in Yoghurt on Chromolith® FastGradient RP-18 endcapped

From our library of Articles, Sigma-Aldrich presents LC/MS Analysis of Artificial Food Colorants Cochineal Red A and Azorubine in Yoghurt on Chromolith® FastGradient RP-18 endcapped
Keywords: Chromatography, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry

LC/MS Analysis of Flavonoids on Chromolith® FastGradient RP-18 endcapped

From our library of Articles, Sigma-Aldrich presents LC/MS Analysis of Flavonoids on Chromolith® FastGradient RP-18 endcapped
Keywords: Chromatography, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry

LC/MS Analysis of Green Tea Components on Chromolith® FastGradient RP-18 endcapped

From our library of Articles, Sigma-Aldrich presents LC/MS Analysis of Green Tea Components on Chromolith® FastGradient RP-18 endcapped
Keywords: Chromatography, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry

LC/MS MS Analysis of Antibiotics Cephalosporin C, Cefaclor and Cephalotin in Human Urine on Chromolith® Performance 50-2mm

From our library of Articles, Sigma-Aldrich presents LC/MS MS Analysis of Antibiotics Cephalosporin C, Cefaclor and Cephalotin in Human Urine on Chromolith® Performance 50-2mm
Keywords: Antibiotics, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Sample preparations

LC/MS MS Analysis of Pesticides in Kidney on Chromolith® Performance 50-2mm

From our library of Articles, Sigma-Aldrich presents LC/MS MS Analysis of Pesticides in Kidney on Chromolith® Performance 50-2mm
Keywords: Environmental, High performance liquid chromatography, Homogenization, Liquid chromatography mass spectrometry, Mass spectrometry, Pesticides, Sample preparations, Solid phase extractions

Monolithic Silica with Bimodal Pore Structure

Thanks to their patented monolithic silica technology, Chromolith® HPLC columns allow you to race through separations with maximum robustness and selectivity—at minimal back pressure.
Keywords: Filtration, High performance liquid chromatography, Precipitation, Sample preparations, Separation, Sol-gel, Solid phase extractions

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