• USA Home
  • SCC065 - HL-1 Cardiac Muscle Cell Line

EMAIL THIS PAGE TO A FRIEND
SCC065 Sigma-Aldrich

HL-1 Cardiac Muscle Cell Line

HL-1 Cardiac Muscle Cell Line has been extensively characterized and is a valuable model system to address questions of cardiac biology at the cellular & molecular levels.

Synonym: HL-1 Cardiomyocyte Cell Line, HL-1 Atrial Muscle Cell Line

  •  eCl@ss 32011203

  •  NACRES NA.81

Purchase

Properties

Related Categories Cell Culture More...
packaging   ≥1X10⁶ cells/vial (SCC065)
application(s)   cell analysis: suitable (cell based assays)
  cell analysis: suitable (cell culture)
biological source   mouse
shipped in   ambient
Quality Assurance   • Each vial contains ≥ 1X106 viable cells.
• Cells tested negative for infectious diseases by the Mouse Essential CLEAR panel PCR panel by Charles River Animal Diagnostic Services.
• Cells are negative for mycoplasma contamination.
• Functional Assay: Fluo-8 calcium dye fluorescent based assay for detecting changes in intracellular calcium

Description

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges {HCompany} to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.

Application

Research Category
Cardiac

Cardiovascular Disease

Toxicity

This product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.

General description

HL-1 Cardiac Muscle Cell Line is an immortalized mouse cardiomyocyte cell line able to continuously divide and spontaneously contract while maintaining a differentiated cardiac phenotype. HL-1 can be serially passaged without losing its differentiated cardiac myocyte phenotype, including morphological, biochemical, and electrophysiological properties. HL-1 cells have been used to study normal cardiomyocyte function with regard to signaling, electrical, metabolic, and transcriptional regulation as well as to address pathological conditions such as hypoxia, hyperglycemia-hyperinsulinemia, apoptosis, and ischemia-reperfusion.

The HL-1 line was derived from AT-1 subcutaneous tumor excised from an adult female C57BL/6J mouse. The parental AT-1 line was originally derived from an atrial tumor growing in a transgenic mouse in which expression of the SV40 large T-antigen was targeted to atrial cardiomyocytes via the atrial natriuretic factor (ANF) promoter.

References:
1. Claycomb, W.C., Lanson, N.A. Jr., Stallworth, B.S., Egeland, D.B., Delcarpio, J.B., Bahinski, A., Izzo, N.J. Jr. (1998). HL-1 cells: a cardiac muscle cell line that contracts and retains phenotypic characteristics of the adult cardiomyocyte. Proc. Natl. Acad. Sci. USA. 95(6): 2979-2984.
2. White, S. M., Constantin, P. E., & Claycomb, W. C. (2004). Cardiac physiology at the cellular level: use of cultured HL-1 cardiomyocytes for studies of cardiac muscle cell structure and function. American Journal of Physiology-Heart and Circulatory Physiology, 286(3), H823-H829.

Cell Line Description

Cardiomyocyte Cells

Storage and Stability

HL-1 Cardiac Muscle Cell Line should be stored in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis

???PDP_SDS_LOT_NUMBER_ERROR_MSG???
Protocols & Articles
Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?