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  • WBAVDFL01 - Immobilon® Block - FL (Fluorescent Blocker)

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WBAVDFL01 Millipore

Immobilon® Block - FL (Fluorescent Blocker)

Fluorescent Detection for Western blotting, dot/slot blotting, mass spectrometry and fluorescence assays

  •  eCl@ss 42029053

  •  NACRES NB.22

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Properties

Related Categories Labware, Manufacturer Browser, Millipore - Manufacturer Browser, Millipore Electrophoresis Supplies
Quality Level   300
packaging   pkg of 500 mL
mfr. no.   bløk
application(s)   western blot: suitable
membrane area   1000  cm2 , membrane coverage
detection method   fluorescent
shipped in   ambient
storage conditions   20 to 25 °C

Description

General description

Immobilon® Block Noise Cancelling Reagents are designed especially for Western blotting applications. This family of protein-free, ready-to-use buffers has been optimized to reduce background levels when using chemiluminescence, fluorescent, or phosphotyrosine detection. Bløk buffers are ideal for use with Immobilon-P Western blotting membranes and the SNAP i.d. system, and are also compatible with standard Western blotting techniques.

Application

For use on immunoblots.

Packaging

Sufficient reagent for 19 mini blots (8x7 cm)

Quality

For optimum results, use Immobilon-P membrane for chemiluminescence (luminol) or visual detection (e.g. TMB), and Immobilon-FL membrane for fluorescent detection applications.

Storage and Stability

Stable for 2 years at 20–25°C

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

Flash Point(F) 
does not flash
Flash Point(C) 
does not flash
Protocols & Articles

Articles

Blocking Reagents for Western Blotting

Immobilon® Block-FL reagent provides exceptional signal-to-noise results. An Immobilon®-FL blots with dilution series of EGF-stimulated A431 lysate were blocked with indicated blocking reagent and in...
Keywords: Amplification, Cell disruption, PAGE

Protocols

Recipe Calculators for Western Blotting Buffers & Solutions

The recipes listed enable the accurate preparation of commonly used solutions for Western blotting, whether you are mixing low volumes for a few experiments or multiple liters for the entire lab to u...
Keywords: Buffers, Detergents, Western blot

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