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10775835001 Roche

Bovine Serum Albumin Fraction V, fatty acid free

from bovine serum

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Properties

Related Categories Albumin, Biochemicals and Reagents, Bovine Serum Albumins, Ethanol Fractionated, Proteins and Derivatives More...
Quality Level   100
biological source   bovine
assay   98.5% (electrophoresis)
form   lyophilized
mol wt   Mr 68 kDa
packaging   pkg of 50 g
mfr. no.   Roche
application(s)   immunofluorescence: suitable
  ligand binding assay: suitable
shipped in   wet ice
storage temp.   2-8°C
Gene Information   bovine ... ALB(280717)

Description

General description

Highest quality for fatty acid and lipid research.
BSA is a single polypeptide chain consisting of about 583 amino acid residues and no carbohydrates. At pH 5-7, it contains 17 intra-chain disulfide bridges and one sulfhydryl group.

Application

Bovine Serum Albumin, Fraction V, fatty acid free is used:
• in lipid research to study lipid metabolism in biological systems
• to identify transport mechanisms in living organisms
• to study ligand binding
• to investigate ionic binding
• in the preparation of solutions during isolation of cardiomyocytes
• as a blocking solution to incubate embryos for immunofluorescence
• in dual polarisation interferometry (DPI) to study the interaction between membrane active peptides and artificial bilayers

Biochem/physiol Actions

The fatty acids that are insoluble in circulating plasma are transported primarily by serum albumin. The human serum albumin and bovine serum albumin share 76% sequence homology. Being the most abundant protein in the circulatory system, it participates in the maintenance of blood pH and carries several endogenous and exogenous compounds, including fatty acids, metal, amino acids, steroids and drugs.

Features and Benefits

• Free from fatty acids according to current quality control procedures (suitable for lipid research)
• High quality
• Low content of heavy metal ions
• Long-term stability

Quality

Purity: ≥98.5 % albumin (electrophoresis), 98% protein
Contaminants: <0.2 mg fatty acids/g, triglycerides not detectable (enzymatic), immunoglobulins not detectable (Ouchterlony), ≤5% H2O,≤30 ppm heavy metals

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles
Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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