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DCTP-RO Roche

dCTP

PCR Grade, sodium salt

Synonym: 2′-Deoxycytidine 5′-triphosphate disodium salt, dCTP-Na2

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Properties

Related Categories 100 mM Solutions, Molecular Biology, PCR/Amplification, dNTPs (Deoxynucleotides)
Quality Level   100
assay   <0.9% (dNDP)
  99% (dNTP)
form   solution
usage   sufficient for 125,000 standard reactions (03732690001)
  sufficient for 31,250 standard reactions (11969021001)
  sufficient for 6,250 standard reactions (11934520001)
packaging   pkg of 1,250 μL (11969021001 [100 mM])
  pkg of 4 × 1,250 μL (03732690001 [4x100 mM])
  pkg of 250 μL (11934520001 [100 mM])
mfr. no.   Roche
concentration   100 mM
shipped in   dry ice
storage temp.   −20°C
SMILES string   [Na+].[Na+].NC1=NC(=O)N(C=C1)[C@H]2C[C@H](O)[C@@H](COP(O)(=O)OP([O-])(=O)OP(O)([O-])=O)O2
InChI   1S/C9H16N3O13P3.2Na/c10-7-1-2-12(9(14)11-7)8-3-5(13)6(23-8)4-22-27(18,19)25-28(20,21)24-26(15,16)17;;/h1-2,5-6,8,13H,3-4H2,(H,18,19)(H,20,21)(H2,10,11,14)(H2,15,16,17);;/q;2*+1/p-2/t5-,6+,8+;;/m0../s1
InChI key   ABWVCNMFYVEBIB-CDNBRZBRSA-L

Description

General description

dCTP, PCR Grade, is a 100 mM clear colorless solution of the sodium salt (pH 8.3). PCR Grade nucleotides from Roche are specially manufactured and purified to the highest possible chemical purity. PCR Grade nucleotides contain at least 99% of the relevant deoxynucleoside-triphosphate (dNTP) and less than 0.9% deoxynucleoside-diphosphate (dNDP).

Application

2′-Deoxycytidine 5′-triphosphate disodium salt is one of the nucleoside triphosphates used for DNA polymerase driven reactions such as polymerase chain reaction, DNA sequencing and other molecular biology methods.

Quality

Function tested in RT-PCR. Tested for absence of contaminating deoxyribonucleases, ribonucleases and nicking activities according to the current Quality Control procedures.

Physical form

solution of dCTP in water (pH 8.3).

Preparation Note

Working solution: We recommend to prepare a deoxynucleotide mix containing dATP, dCTP, dGTP, dTTP (10 mM, each); (e.g., for the preparation of 100 μl nucleotide mix add 10 μl of dATP, dCTP, dGTP, dTTP (each) to 60 μl Water, PCR Grade).

Other Notes

For general laboratory use.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Protocols & Articles

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