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Expand High FidelityPLUS PCR System, dNTPack

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Properties

Related Categories Biochemicals and Reagents, Fluorescent Enzyme Substrates, Fluorescent Enzyme Substrates for Immunoassays, Fluorescent Probes, Labels, Particles and Stains
Quality Level   100
usage   sufficient for ≤125 reactions (04743725001)
  sufficient for ≤500 reactions (04743733001)
packaging   pkg of 125 U (04743725001)
  pkg of 500 U (04743733001 [2x250 U])
mfr. no.   Roche
parameter   72 °C optimum reaction temp.
shipped in   dry ice
storage temp.   −20°C

Description

General description

The Expand High FidelityPLUS PCR System is a blend of Taq DNA Polymerase and a thermostable proofreading protein that lacks polymerase activity.
This enzyme blend is designed to amplify fragments up to 5 kb from all types of DNA with high yield and fidelity.
In addition, this enzyme blend also incorporates dUTP and, in combination with Uracil-DNA Glycosylase, can be used to safeguard PCR reactions from cross contamination.
The fidelity of DNA synthesis with the Expand High FidelityPLUS PCR System shows a 2-fold increase compared to the fidelity obtained with the Expand High Fidelity PCR System and a 6-fold increase compared to the fidelity obtained with Taq DNA Polymerase.
These characteristics make the Expand High FidelityPLUS PCR System blend the product of choice if a larger number of targets need to be amplified at the same time with high yield in combination with improved accuracy. In addition, the product can be used for the efficient labeling of DNA fragments with radioactive or non-radioactive modified nucleotides.

Application

• The Expand High FidelityPLUS PCR System is designed for the robust amplification of fragments up to 5 kb from all types of DNA with outstanding yield, specificity, sensitivity, and accuracy. This convenient dNTPack is the product of choice for the development of a new amplification assay or the optimization of an existing assay. PCR
• RT-PCR
• Primer extension
• Carryover prevention
It has been used in the ultradeep pyrosequencing methods, inverse PCR and to measure the repeat length of CGG sequences.

Twofold higher yield and threefold greater fidelity are obtained compared to Taq DNA Polymerase. Use Expand High FidelityPLUS PCR System for:
• PCR up to 5kb
• RT-PCR up to 5kb

Features and Benefits

Expand High FidelityPLUS PCR System is a mixture of Taq DNA polymerase and a DNA polymerase with proofreading activity. The Expand High FidelityPLUS PCR System shows a twofold increase in fidelity compared to the Expand High Fidelity PCR System, and a sixfold increase compared to Taq DNA Polymerase.
By incorporating dUTP, Uracil-DNA Glycosylase can be used to prevent PCR product carryover. Use this kit for efficient labeling of DNA with radioactive or non-radioactive nucleotides. It is optimized for PCR of DNA fragments up to 5kb.
Use 2.5U for a standard 50μl PCR.

• Safeguard against cross-contamination.
This is the choice for large numbers of amplifications.
• Obtain sixfold higher fidelity and higher yields,
compared to Taq DNA Polymerase.
• Efficiently label DNA.
Use this kit to label DNA with radioactive or nonradioactive nucleotides.
• Cost-effective.
Use the convenient solution of premixed PCR grade dNTPs.

Packaging

1 kit containing 5 components

Quality

Each lot is PCR function-tested using human genomic DNA and EPO primers specific for a 1.8kb PCR product, and tPA primers specific for a 4.8kb PCR product.

Unit Definition

Volume Activity: 5 U/μl

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

Use of this product is covered by US patent claims and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patent claims require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

Expand is a trademark of Roche

Kit component only

Description

Product #

Add to Cart

Expand High FidelityPLUS Enzyme Blend 5 U/μl    
Expand High FidelityPLUS Reaction Buffer, with 7.5 mM MgCl2 5x concentrated    
Expand High FidelityPLUS Reaction Buffer, without MgCl2 5x concentrated    
MgCl2 Stock Solution 25 mM    
PCR Nucleotide Mix    
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles

Articles

Expand™ High FidelityPLUS PCR System, dNTPack Troubleshooting

ELEVATED ERROR RATES The typical error rate (fidelity) of Taq DNA Polymerase, determined by the lacI-based PCR fidelity assay, ranges from 1 × 10-5 to 2.5 × 10-5. Roche has determined an error rate o...
Keywords: Amplification, Polymerase chain reaction, Solvents, Titrations

Roche PCR Reagents and PCR Protocols

For additional information on Hot Start PCR technology, protocols, and to download our free Hot Start PCR eBook, please visit our Hot Start PCR resource guide.
Keywords: Amplification, Cloning, Gas chromatography, Gene expression, Polymerase chain reaction, Polymerase chain reaction - quantitative, Purification, Sequencing, Transcription

Peer-Reviewed Papers
15

References

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