01769 Sigma-Aldrich

Folic acid

tested according to Ph Eur

Synonym: Acidum folicum, PteGlu, Pteroyl-L-glutamic acid, Vitamin M

  • CAS Number 59-30-3

  • Empirical Formula (Hill Notation) C19H19N7O6

  • Molecular Weight 441.40

  •  Beilstein/REAXYS Number 100781

  •  EC Number 200-419-0

  •  MDL number MFCD00079305

  •  eCl@ss 34058014

  •  PubChem Substance ID 329747356



Related Categories Amino Acids, Biochemicals and Reagents, Core Bioreagents, Folic Acid, Research Essentials,
biological source   synthetic
Agency/Method   tested according to Ph Eur
assay   96.0-102.0% anhydrous basis
form   powder
color   yellow
mp   250 °C
solubility   cold water: very slightly soluble 0.0016 mg/ml at 25 degrees C
  boiling water: soluble 1%
  1-butanol: partially soluble
  acetone: insoluble
  benzene: insoluble
  chloroform: insoluble
  diethyl ether: insoluble
  dilute HCl: soluble (Hot)
  ethanol: partially soluble
  methanol: slightly soluble
  sulfuric acid: soluble
storage temp.   2-8°C
SMILES string   NC1=NC(=O)c2nc(CNc3ccc(cc3)C(=O)N[C@@H](CCC(O)=O)C(O)=O)cnc2N1
InChI   1S/C19H19N7O6/c20-19-25-15-14(17(30)26-19)23-11(8-22-15)7-21-10-3-1-9(2-4-10)16(29)24-12(18(31)32)5-6-13(27)28/h1-4,8,12,21H,5-7H2,(H,24,29)(H,27,28)(H,31,32)(H3,20,22,25,26,30)/t12-/m0/s1



Folic acid was injected in control and manifest diabetic pregnant rats for studying whether folic acid supplementation can moderate diabetes and glucose-induced dysmorphogenesis in rat embryos. It can also be used in the synthesis of N10-trifluoroacetylpteroic acid.

Biochem/physiol Actions

Folic acid also referred to as folate, vitamin M or vitamin Bc is a form of water-soluble vitamin B9. Folic acid therapy can reduce elevated plasma levels of arginine and asymmetric dimethylarginine (ADMA) in hyperhomocysteinaemic subjects. Folic acid used as a food supplement in early pregnancy can diminish the risk of neural tube defects.

Preparation Note

Folic acid dissolves in hot dil HCl, H2SO4 and about 1% boiling water. It is slightly soluble in methanol and shows very slight solubility in cold water (0.0016 mg/ml at 25°C). Additionally, the product appreciably dissolves in ethanol and butanol and has relative solubility in acetic acid, phenol, pyridine, solutions of alkali hydroxides and carbonates. It is insoluble in acetone, benzene, ether and chloroform.

Safety & Documentation

Safety Information

Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 


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