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114413 Sigma-Aldrich

Bromothymol Blue

ACS reagent, Dye content 95 %

Synonym: 3′,3″-Dibromothymolsulfonphthalein

  • CAS Number 76-59-5

  • Empirical Formula (Hill Notation) C27H28Br2O5S

  • Molecular Weight 624.38

  •  Beilstein Registry Number 373934

  •  EC Number 200-971-2

  •  MDL number MFCD00005872

  •  PubChem Substance ID 24847171

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Properties

Related Categories B, Cell Biology, Hematology and Histology, Stains & Dyes, A to Z, Stains and Dyes More...
grade   ACS reagent
InChI Key   NUHCTOLBWMJMLX-UHFFFAOYSA-N
form   powder
composition   Dye content, 95%
visual transition interval   6.0 - 7.6, yellow to blue (passes test)
mp   200-202 °C (lit.)
absorption   extinction/279-285 nm, methanol ≥9000
  extinction/326-332 nm, methanol ≥8000
  extinction/419-425 nm, methanol ≥17000
  λmax 420 nm

Description

Packaging

5, 25 g in glass bottle

Application

Bromothymol Blue (BTB) is a pH indicator (pKa approx. 7.1) that is used as a biological slide vital stain to define cell walls or nuclei under the microscope. BTB is also a component of several bacterial growth and detection substrates such as cystine lactose electrolyte deficient (CLED/BROLACIN); polymyxin pyruvate egg yolk mannitol bromothymol blue (PEMBA) and MacConkey agar.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin


LC-MS Grade Solvents and Reagents

Biomedical Applications
Protocols & Articles
Peer-Reviewed Papers
15

References

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