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199664 Sigma-Aldrich

Sudan Black B

certified by the Biological Stain Commission

Synonym: Ceres black BN, Fat Black HB, Solvent Black 3

  • CAS Number 4197-25-5

  • Empirical Formula (Hill Notation) C29H24N6

  • Molecular Weight 456.54

  •  Colour Index Number 26150

  •  Beilstein Registry Number 723248

  •  EC Number 224-087-1

  •  MDL number MFCD00006919

  •  PubChem Substance ID 24851932

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Properties

Related Categories Cell Biology, Certified Stains & Dyes, Hematology and Histology, S, Stains & Dyes, A to Z,
grade   certified by the Biological Stain Commission
InChI Key   YCUVUDODLRLVIC-VPHDGDOJSA-N
form   powder
mp   120-124 °C (lit.)
absorption   λmax 415 nm (2nd)
  λmax 598 nm
  extinction/596-603 nm, ethanol ≥20000

Description

Packaging

25 g in glass bottle

Application

Sudan Black B has been used in brain BLAQ (Block Lipids and Aldehyde Quench) technique and is used in lipid detection.

Biochem/physiol Actions

Sudan Black B has been used to stain phospholipids and intracellular lipids. Staining pattern is almost similar to myeloperoxidase reaction, with positive staining of granulocytic cells, eosinophils and weak monocytic staining.It doesn’t stain lymphocytes. Sudan Black B can be used to stain older blood or bone marrow smears, but the stain slightly fades over time. It is considered to be a most sensitive and specific reagent for lipid staining. Sudan Black B stains phospholipids gray and neutral fats blue-black.

Suitability

Certified as a fat staining using the Churukian variant of the Lillie-Ashburn supersaturated isopropanol method. Sudan III is substituted for the oil red O in the procedure. Burdon′s stain for bacterial lipid.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
SD4431500

Documents

Certificate of Analysis

Certificate of Origin


Milli-Q® Water Purification Solutions

Biomedical Applications
Protocols & Articles
Peer-Reviewed Papers
15

References

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