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30787 Millipore

Deoxyribonuclease Test Agar

for microbiology

  •  NACRES NA.85

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Properties

Related Categories A - M, All Food Control Media (alphabetical), All Solid Media/Agars, Biochemical Identification Media by Test, Biochemical Identification Media, A to Z,
Quality Level   100
grade   for microbiology
form   powder
shelf life   limited shelf life, expiry date on the label
composition   agar, 15 g/L
  deoxyribonucleic acid, 2 g/L
  sodium chloride, 5 g/L
  tryptose, 20 g/L
application(s)   microbiological culture: suitable
  pathogen testing: suitable
final pH   7.3±0.2 (37 °C)
Featured Industry   Clinical Testing
Environmental
Food and Beverages
suitability   nonselective and differential for Serratia spp.
  nonselective and differential for Staphylococcus spp.
  nonselective and differential for Streptococcus spp.

Description

General description

Deoxyribonuclease Test Agar is recommended for the detection of deoxyribonuclease activity of bacteria and fungi, and especially for identification of pathogenic Staphylococci.
Bacteria are streaked on to the surface of the agar medium and incubated. The growth on the surface of the agar is then flooded with 1N hydrochloric acid. Polymerised DNA precipitates in the presence of 1N HCl and makes the medium opaque. If the organisms produce DNase enzymes, in sufficient quantity to hydrolyse the DNA, then clear zones are seen around the colonies.

Application

for the detection of deoxyribonuclease activity of bacteria and fungi, and especially for identification of pathogenic Staphylococci

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Microbiology Introduction

An initial aim of all microbiologists is the reproducible growth of their microbial cultures, no matter whether the microorganisms are of natural origin or have been genetically engineered by man. Re...
Keywords: Absorption, Aerobic, Anaerobic, Antibiotics, Antimicrobials, Buffers, Culture media, Diffusion, Environmental, Filtration, Genetic, Growth factors, Metabolism, Phase transitions, Precipitation, Reductions, Respiration, Sample preparations, Vitamins

Microbiology Theory: Media Preparation

Microorganisms need nutrients, a source of energy and certain environmental conditions in order to grow and reproduce. In the natural environment, microbes have adapted to the habitats most suitable ...
Keywords: Culture media, Digestions, Environmental, Filtration, Growth factors, Indicators, Microbiology, Phase transitions, Precipitation, Vitamins

Reliable Testing Solutions & Media for Detection of Staphylococcus Aureus: a Spreading Bacteria

Staphylococcus aureus is frequently a part of our skin flora but is also a cause of a broad range of illnesses. Current studies report a remarkable increase of Methicillin Resistant Staphylococcus au...
Jvo Siegrist
AnalytiX Volume 10 Article 2
Keywords: Aerobic, Antibiotics, Clinical, Environmental, Fermentation, Food & Beverage, Microbiology, Respiration

Peer-Reviewed Papers
15

References

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