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38266 Millipore

Ferrioxamine E from Streptomyces antibioticus

for microbiology, ≥95%

Synonym: 1,12,23-Trihydroxy-1,6,12,17,23,28-hexaazacyclotritriacontane-2,5,13,16,24,27-hexone Iron(III) complex

  • CAS Number 20008-20-2

  • Empirical Formula (Hill Notation) C27H45FeN6O9

  • Molecular Weight 653.53

  •  MDL number MFCD07785179

  •  PubChem Substance ID 329755756

  •  NACRES NA.85

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Properties

Related Categories Base Ingredients for Media, Microbiological Culture Media, Microbiology, Other Additives, Supplements More...
Quality Level   200
grade   for microbiology
assay   ≥95% (HPLC)
  ≥95%
form   powder
loss   ≤6% loss on drying
Featured Industry   Environmental
Food and Beverages
storage temp.   2-8°C
SMILES string   [Fe+3].[O-]N1CCCCCNC(=O)CCC(=O)N([O-])CCCCCNC(=O)CCC(=O)N([O-])CCCCCNC(=O)CCC1=O
InChI   1S/C27H45N6O9.Fe/c34-22-10-14-26(38)32(41)20-8-3-6-18-30-24(36)12-15-27(39)33(42)21-9-2-5-17-29-23(35)11-13-25(37)31(40)19-7-1-4-16-28-22;/h1-21H2,(H,28,34)(H,29,35)(H,30,36);/q-3;+3
InChI key   MZFKJKOHYACYNT-UHFFFAOYSA-N

Description

General description

Scientifically proven supplement for culture media improves detection of Salmonella, Enterobacter sakazakii and Yersinia enterocolitica .

Ferrioxamine E from Streptomyces antibioticum is a siderophore which facilitates the supply of iron (III), an essential trace element, to bacteria involved in food poisoning, including Salmonella, Enterobacter saka-zakii and Yersinia enterocolitica. It promotes rapid growth by reducing the lag phase in culture media and reactivates dormant bacteria. Use of ferrioxamine E can permit rapid detection of low bacterial cell counts, even from dry products like spices and tea after long storage periods. It is an essential component of quality control efforts in the food industry.

Ferrioxamine E is often used in Buffered Peptone Water, the medium recommended by the ISO-Norms for Enterobacteriacea. Ferrioxamine E also improves the motility of Salmonella, which helps to improve the identification by semisolid selective motility media like SIM, MRSV, DIASSALM or SMS. Ferrioxamine E does not improve growth of Escherichia coli, Shigella, Proteus, Providencia and Morganella species; a feature that makes it a semi-selective compound. The recommended concentration of Ferrioxamine E to promote bacterial growth is between 5 and 200 ng/mL

The ferrioxamines are basically metabolic products produced by the actinomycetes, and are characterised as ferrioxamines A, B, C, D 1, D2, E, F and G. Generally all ferrioxamines are trihydroxamates and the deferrioxamines are either linear or cyclic (D2 and E).

Application

Supplementation of pre-enrichment broth and enrichment broth media for improved recovery of Salmonella from artificially or naturally contaminated foods

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Ferrioxamine E

Ferrioxamine E Scientifically proven supplement for culture media improves detection of Salmonella, Enterobacter sakazakii and Yersinia enterocolitica
Jvo Siegrist
AnalytiX Volume 5 Article 5
Keywords: Culture media, Food & Beverage

Microbiological Control of Spices and Herbs

Cultures around the world rely on herbs and spices to add flavour and zest to food. Many spices, however, contain very high numbers of bacteria, making them a potent source for food spoilage and path...
Jvo Siegrist
AnalytiX Volume 10 Article 1
Keywords: Growth factors, Microbiology

Microbiology Introduction

An initial aim of all microbiologists is the reproducible growth of their microbial cultures, no matter whether the microorganisms are of natural origin or have been genetically engineered by man. Re...
Keywords: Absorption, Aerobic, Anaerobic, Antibiotics, Antimicrobials, Buffers, Culture media, Diffusion, Environmental, Filtration, Genetic, Growth factors, Metabolism, Phase transitions, Precipitation, Reductions, Respiration, Sample preparations, Vitamins

Microbiology Theory: Media Preparation

Microorganisms need nutrients, a source of energy and certain environmental conditions in order to grow and reproduce. In the natural environment, microbes have adapted to the habitats most suitable ...
Keywords: Culture media, Digestions, Environmental, Filtration, Growth factors, Indicators, Microbiology, Phase transitions, Precipitation, Vitamins

Viable but Nonculturable Bacteria

The expression “viable but nonculturable” (VNC) bacteria, describes cells that cannot normally be cultured. However this makes little sense, when one considers that the demonstration of culturability...
Jvo Siegrist
Microbiology Focus Edition 1.4
Keywords: Environmental, Gene expression, Metabolic Pathways

Peer-Reviewed Papers
15

References

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