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38497 Millipore

Nitrate Reagent A

for microbiology

Synonym: 1-Naphthylamine solution

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Properties

Related Categories Identification Tests & Reagents, Microbiology, Test Kits More...
Quality Level   100
grade   for microbiology
shelf life   limited shelf life, expiry date on the label
composition   acetic acid 5 N, 1000 mL
  α-naphthylamine, 5 g
application(s)   microbiology: suitable (microbial identification)
Featured Industry   Agriculture
Clinical Testing
Environmental
Food and Beverages
Pharmaceutical
suitability   bacteria
InChI   1S/C10H9N/c11-10-7-3-5-8-4-1-2-6-9(8)10/h1-7H,11H2
InChI key   RUFPHBVGCFYCNW-UHFFFAOYSA-N

Description

General description

α-Naphtylamine and sulfanilic acid are used for detection of nitrate reduction by bacteria. Organisms containing nitrate reductase reduce nitrate to nitrite, which forms a diazonium salt with sulfanilic acid and reacts with α-naphtylamine to form a red azo dye.

Safety & Documentation

Safety Information

Symbol 
GHS05  GHS05
Signal word 
Danger
Hazard statements 
RIDADR 
UN 2790C 8 / PGIII
WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Differentiation of Escherichia coli from coliforms

In August 2008, the discovery of E. coli-contaminated beef in the United States prompted a nationwide recall of beef. The source turned out to be one supplier that had a history of contamination of i...
Jvo Siegrist
AnalytiX Volume 8 Article 5
Keywords: Decarboxylations, Fermentation, Genetic, Indicators, Microbiology, Reductions

Identification of Microorganisms Based on Color

For microbiologists the most fundamental stain was developed in 1884 by the Danish bacteriologist Hans Christian Gram. The Gram Stain allows the determination of morphology, dividing bacteria into tw...
Jvo Siegrist
Microbiology Focus Edition 1.4, Product Manager Microbiology.... ivo.siegrist@sial.com
Keywords: Antibiotics, Enzyme activity, Fermentation, Gram stain, Help, Indicators, Reductions

Microbiology Introduction

An initial aim of all microbiologists is the reproducible growth of their microbial cultures, no matter whether the microorganisms are of natural origin or have been genetically engineered by man. Re...
Keywords: Absorption, Aerobic, Anaerobic, Antibiotics, Antimicrobials, Buffers, Culture media, Diffusion, Environmental, Filtration, Genetic, Growth factors, Metabolism, Phase transitions, Precipitation, Reductions, Respiration, Sample preparations, Vitamins

On the Trail of Campylobacter

The Campylobacter is one of the leading causes of human gastroenteritis. Common Campylobacter species C. jejuni, C. coli, and C. lari are responsible for most cases of campylobacteriosis1,2. However,...
Jvo Siegrist
AnalytiX Volume 8 Issue 1
Keywords: Antibiotics, Cancer, Citric Acid Cycle, Gram stain, Metabolism, Microbiology, Scanning electron microscopy

US EPA Method 8270 (Appendix IX): GC Analysis of Semivolatiles on Equity®-5 (30 m x 0.25 mm I.D., 0.50 μm)

From our library of Articles, Sigma-Aldrich presents US EPA Method 8270 (Appendix IX): GC Analysis of Semivolatiles on Equity®-5 (30 m x 0.25 mm I.D., 0.50 μm)
Keywords: Chromatography, Gas chromatography, Mass selective detector, Purification

US EPA Method 8270 (Appendix IX): GC Analysis of Semivolatiles on SLB®-5ms (30 m x 0.25 mm I.D., 0.50 μm)

From our library of Articles, Sigma-Aldrich presents US EPA Method 8270 (Appendix IX): GC Analysis of Semivolatiles on SLB®-5ms (30 m x 0.25 mm I.D., 0.50 μm)
Keywords: Chromatography, Gas chromatography, Purification

Vibrio Detection

Vibrios are motile, curved or comma-shaped bacilli and have a single polar flagella with sheet proteins. They are often found in open water, freshwater and saltwater. Vibrios are facultative aerobe a...
Jvo Siegrist
Microbiology Focus Edition 1.3
Keywords: Dehydration reaction, Diagnostic, Fermentation, Indicators, Metabolism, Microbiology

Peer-Reviewed Papers
15

References

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