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53286 Millipore

Brain Heart Infusion Broth

for microbiology

Synonym: BHI Broth, Brain Heart Broth

  •  NACRES NA.74

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Properties

Related Categories A - M, All Dairy Media (alphabetical), All Food Control Media (alphabetical), All Liquid Media/Broths, Bacteria (General Media),
Quality Level   100
grade   for microbiology
form   powder
shelf life   limited shelf life, expiry date on the label
composition   beef heart (infusion from 250g), 5 g/L
  calf brains (infusion from 200g), 12.5 g/L
  disodium hydrogen phosphate, 2.5 g/L
  D(+)-glucose, 2 g/L
  peptone, 10 g/L
  sodium chloride, 5 g/L
final pH   7.4±0.2 (25 °C)
Featured Industry   Clinical Testing
Environmental
Food and Beverages
Life Science and Biopharma
Pharmaceutical
suitability   Haemophilus spp.
  nonselective for Bacteroides spp.
  nonselective for Candida spp.
  nonselective for Escherichia coli
  nonselective for Neisseria spp.
  nonselective for Pseudomonas spp.
  nonselective for Staphylococcus spp.
  nonselective for Streptococcus spp.
  nonselective for Yersinia spp.
  nonselective for bacteria (General Media)
  nonselective for coliforms

Description

Application

Brain Heart Infusion Broth is recommended for the cultivation of fastidious pathogenic microorganisms. Brain Heart Infusion Broth has been used as culture medium forStreptococcus pneumonia, Listeria monocytogenes, Leishmania promastigotes and Clostridium difficile.

Other Notes

Production of 12α-hydroxysteroid dehydrogenase from Clostridium group P

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Microbiological Control of Spices and Herbs

Cultures around the world rely on herbs and spices to add flavour and zest to food. Many spices, however, contain very high numbers of bacteria, making them a potent source for food spoilage and path...
Jvo Siegrist
AnalytiX Volume 10 Article 1
Keywords: Growth factors, Microbiology

Microbiology Introduction

An initial aim of all microbiologists is the reproducible growth of their microbial cultures, no matter whether the microorganisms are of natural origin or have been genetically engineered by man. Re...
Keywords: Absorption, Aerobic, Anaerobic, Antibiotics, Antimicrobials, Buffers, Culture media, Diffusion, Environmental, Filtration, Genetic, Growth factors, Metabolism, Phase transitions, Precipitation, Reductions, Respiration, Sample preparations, Vitamins

Microbiology Theory: Media Preparation

Microorganisms need nutrients, a source of energy and certain environmental conditions in order to grow and reproduce. In the natural environment, microbes have adapted to the habitats most suitable ...
Keywords: Culture media, Digestions, Environmental, Filtration, Growth factors, Indicators, Microbiology, Phase transitions, Precipitation, Vitamins

Pseudomonas Media and Tests

Pseudomonas are motile (one or more polar flagella), rod shaped and aerobe Gram-negative bacteria. They are found almost everywhere, in soil, water, plants and animals. In most cases it is not pathog...
Jvo Siegrist, Product Manager Microbiology, ivo.siegrist@sial.com
Analytix Volume 2007 Article 5
Keywords: Anaerobic, Antifungals, Biochemistry, Cosmetics, Diagnostic, Environmental, Fermentation, Glycolysis, Metabolism, Metabolites, Microbiology, Pentose phosphate pathway, Pharmaceutical, Respiratory

Streptococci- Overview of Detection, Identification, Differentiation and Cultivation Techniques

Streptococci are non-motile, microaerophilic, Grampositive spherical bacteria (cocci). They often occur as chains or pairs and are facultative or strict anaerobes. Streptococci give a negative catala...
By Jvo Siegrist, Product Manager Microbiology, ivo.siegrist@sial.com
AnalytiX Volume 7 Article 3
Keywords: Cell division, Diagnostic, Phosphorylations

Yersinia Diagnostic

Yersinia are rod-shaped, Gram-negative facultative anaerobic bacteria. They exhibit fermentative metabolism and are oxidasenegative, mannitol-positive. A psychrophilic organism, Yersinia survives and...
Ivo Siegrist
Analytix Volume 7 Article 5
Keywords: Anaerobic, Clinical, Diagnostic, Metabolism, Microbiology

Peer-Reviewed Papers
15

References

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