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77187 Millipore

Peptone Water, phosphate-buffered

for microbiology

Synonym: Buffered Peptone Water, Tryptone Phosphate Water

  •  NACRES NA.85

Purchase

Properties

Related Categories All Dairy Media (alphabetical), All Food Control Media (alphabetical), All Liquid Media/Broths, Bacteria (General Media), Biochemical Identification Media by Test,
Quality Level   100
grade   for microbiology
form   powder
shelf life   limited shelf life, expiry date on the label
composition   disodium hydrogen phosphate, 3.5 g/L
  peptone, 10 g/L
  potassium dihydrogen phosphate, 1.5 g/L
  sodium chloride, 5 g/L
final pH   7.2±0.2 (25 °C)
Featured Industry   Environmental
Food and Beverages
suitability   Providencia spp.
  nonselective for Citrobacter spp.
  nonselective for Cronobacter spp.
  nonselective for Escherichia coli
  nonselective for Klebsiella spp.
  nonselective for Proteus spp.
  nonselective for Pseudomonas spp.
  nonselective for Salmonella spp.
  nonselective for Shigella spp.
  nonselective for Staphylococcus spp.
  nonselective for Streptococcus spp.
  nonselective for Yersinia spp.
  nonselective for bacteria (General Media)
  nonselective for coliforms
  nonselective for enterobacteriaceae

Description

General description

Buffered peptone water being suitable as first diluent is helpful since the same homogenate can be used for detection of other microorganisms. Pre-enrichment of the culture media is performed using buffered peptone water to increase target concentration and improve sensitivity.

Application

Buffered peptone water may be used to homogenize poultry samples. It may also be used in microbial culture of Staphylococcus aureus and followed by GC-MS analysis to determine the chemical composition of galangal extraxt and HPLC analysis to isolate compounds of Indian gooseberry extract.

Other Notes

Studies with salmonellae

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Chronobacter spp. Classic and New Detection Methods

Cronobacter is a rod-shaped, motile and facultatively-anaerobic bacteria of the family Enterobacteriaceae. Originally Cronobacter spp. were listed as yellow-pigmented Enterobacter cloacae (see Table ...
Jvo Siegrist
Microbiology Focus Edition 1.2
Keywords: Absorption, Anaerobic, Diagnostic, Environmental, Enzyme-linked immunosorbent assay, Food Safety, Genetic, Microbiology, Nutrition, Polymerase chain reaction, Proteomics, Vitamins

Culture Media and Tests for Beverage Testing

Spoilage organism testing is a crucial step within the beverage industry to enable the safe release of drinks to the market. Requirements can vary from industry to industry, depending on different sp...
Keywords: Culture media, Fermentation, Magnetic resonance spectroscopy

Enterobacteriaceae in Food

From our library of Articles, Sigma-Aldrich presents Enterobacteriaceae in Food

Microbiology Introduction

An initial aim of all microbiologists is the reproducible growth of their microbial cultures, no matter whether the microorganisms are of natural origin or have been genetically engineered by man. Re...
Keywords: Absorption, Aerobic, Anaerobic, Antibiotics, Antimicrobials, Buffers, Culture media, Diffusion, Environmental, Filtration, Genetic, Growth factors, Metabolism, Phase transitions, Precipitation, Reductions, Respiration, Sample preparations, Vitamins

Microbiology Theory: Media Preparation

Microorganisms need nutrients, a source of energy and certain environmental conditions in order to grow and reproduce. In the natural environment, microbes have adapted to the habitats most suitable ...
Keywords: Culture media, Digestions, Environmental, Filtration, Growth factors, Indicators, Microbiology, Phase transitions, Precipitation, Vitamins

Pseudomonas Media and Tests

Pseudomonas are motile (one or more polar flagella), rod shaped and aerobe Gram-negative bacteria. They are found almost everywhere, in soil, water, plants and animals. In most cases it is not pathog...
Jvo Siegrist, Product Manager Microbiology, ivo.siegrist@sial.com
Analytix Volume 2007 Article 5
Keywords: Anaerobic, Antifungals, Biochemistry, Cosmetics, Diagnostic, Environmental, Fermentation, Glycolysis, Metabolism, Metabolites, Microbiology, Pentose phosphate pathway, Pharmaceutical, Respiratory

Streptococci- Overview of Detection, Identification, Differentiation and Cultivation Techniques

Streptococci are non-motile, microaerophilic, Grampositive spherical bacteria (cocci). They often occur as chains or pairs and are facultative or strict anaerobes. Streptococci give a negative catala...
By Jvo Siegrist, Product Manager Microbiology, ivo.siegrist@sial.com
AnalytiX Volume 7 Article 3
Keywords: Cell division, Diagnostic, Phosphorylations

Yersinia Diagnostic

Yersinia are rod-shaped, Gram-negative facultative anaerobic bacteria. They exhibit fermentative metabolism and are oxidasenegative, mannitol-positive. A psychrophilic organism, Yersinia survives and...
Ivo Siegrist
Analytix Volume 7 Article 5
Keywords: Anaerobic, Clinical, Diagnostic, Metabolism, Microbiology

Peer-Reviewed Papers
15

References

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