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88597 Millipore

Hydrogen peroxide solution

3%, for microbiology

Synonym: Catalase Test

  • CAS Number 7722-84-1

  • Empirical Formula (Hill Notation) H2O2

  • Molecular Weight 34.01

  •  Beilstein/REAXYS Number 3587191

  •  MDL number MFCD00011333

  •  PubChem Substance ID 329769664

  •  NACRES NA.85



Related Categories Biochemical Reagents for Identification, Identification Tests & Reagents, Microbiology More...
Quality Level   100
grade   for microbiology
vapor pressure   23.3 mmHg ( 30 °C)
assay   2.8-3.2% (redox titration)
shelf life   limited shelf life, expiry date on the label
concentration   1-5%
application(s)   microbiology: suitable (microbial identification)
mp   0.0 °C
density   1.000 g/cm3
Featured Industry   Clinical Testing
Food and Beverages
suitability   Pneumococcus spp.
  Staphylococcus spp.
SMILES string   OO
InChI   1S/H2O2/c1-2/h1-2H


General description

A reagent to detect the enzymes catalase and peroxidase. Catalase and peroxidase is found in most aerobe bacteria. They catalyse the breakdown of hydrogem peroxide with the release of free oxygen (resulting in bubbles).The classical test for differentiating staphylococci (positive) from streptococci (negative).To observe the action of the enzymes, catalase reagent is added to a pure bacterial culture. Any immediate bubbling is indicative of a positive result since oxygen is a by-product of hydrogen peroxide decomposition. The test is also useful in differentiating Gram positive bacilli.


Hydrogen peroxide solution has been used:
• to stimulate Src homology region 2 domain-containing phosphatase-1 (SHP-1) oxidation in thymocytes
• for the induction of apoptosis in cultured human microvascular endothelial cells
• as a substrate for superoxide dismutase

Safety & Documentation

Safety Information

Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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