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B4501 Sigma-Aldrich

Biotin

≥99% (HPLC), lyophilized powder

Synonym: D-Biotin, Bios II, Coenzyme R, Vitamin B7, Vitamin H

  • CAS Number 58-85-5

  • Empirical Formula (Hill Notation) C10H16N2O3S

  • Molecular Weight 244.31

  •  Beilstein Registry Number 86838

  •  EC Number 200-399-3

  •  MDL number MFCD00005541

  •  eCl@ss 34058011

  •  PubChem Substance ID 24891778

Purchase

Description

Packaging

1, 5, 10, 25 g in poly bottle

100, 500 mg in poly bottle

Application

Biotin may be used to elute proteins from avidin/streptavidin resins.
• It has been used for culturing of oligodendrocytes.
• It has been used as a vitamin supplement for the growth of Bacillus species.
• It has been used for blocking endogenous biotin during immunohistology procedures.

Biochem/physiol Actions

Biotin is a small molecule and its presence with biologically active macromolecules does not affect the biological function of the macromolecule. The biotin-(strept)avidin system are required for various applications. Biotin also works as a cofactor of carboxylating enzymes, such as pyruvate carboxylase. It catalyzes the formation of oxaloacetate from pyruvate and CO2. Biotin conjugation with avidin or streptavidin helps in linking the target molecule (antibody, nucleotide, protein A etc.) with the labeling system (enzyme, fluorescent, chemiluminescent probes). This complex is used for many detection systems, such as immunoassays, DNA hybridization assays, immunohistochemistry and flow-cytometry. This approach is also applied for the purification and characterization of various molecules of interest.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1
RTECS 
XJ9088200

Documents

Certificate of Analysis

Certificate of Origin


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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

MSMLS Online Plate Map

Metabolite descriptors included with the software download upon purchase of the product include: Name, Parent CID, molecular formula, molecular weight, CAS, ChEBI, HMDB ID, PubChem Compound and Subst...
Keywords: Mass spectrometry, Metabolites

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抗体基础知识

免疫球蛋白(Ig)由B淋巴细胞产生并分泌到血浆中。单体形式的Ig分子是一个分子量约为150 kDa的糖蛋白,且其结构差不多类似字母Y。Ig单体的基本结构(图1)包括由两个二硫键连接的两个相同的半体。每个半体都具有一个约50 kDa的重链和一个约25 kDa的轻链,两者在轻链羧基末端附近通过二硫键连接在一起。重链可分为Fc部分和Fab部分,前者位于羧基末端(Y的基座),后者则位于氨基末端(Y的臂)...
Keywords: Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Rheumatology

Peer-Reviewed Papers
15

References

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