B4501 Sigma-Aldrich


≥99% (HPLC), lyophilized powder

Synonym: D-Biotin, Bios II, Coenzyme R, Vitamin B7, Vitamin H

  • CAS Number 58-85-5

  • Empirical Formula (Hill Notation) C10H16N2O3S

  • Molecular Weight 244.31

  •  Beilstein Registry Number 86838

  •  EC Number 200-399-3

  •  MDL number MFCD00005541

  •  eCl@ss 34058011

  •  PubChem Substance ID 24891778




1, 5, 10, 25 g in poly bottle

100, 500 mg in poly bottle


Biotin may be used to elute proteins from avidin/streptavidin resins.
• It has been used for culturing of oligodendrocytes.
• It has been used as a vitamin supplement for the growth of Bacillus species.
• It has been used for blocking endogenous biotin during immunohistology procedures.

Biochem/physiol Actions

Biotin is a small molecule and its presence with biologically active macromolecules does not affect the biological function of the macromolecule. The biotin-(strept)avidin system are required for various applications. Biotin also works as a cofactor of carboxylating enzymes, such as pyruvate carboxylase. It catalyzes the formation of oxaloacetate from pyruvate and CO2. Biotin conjugation with avidin or streptavidin helps in linking the target molecule (antibody, nucleotide, protein A etc.) with the labeling system (enzyme, fluorescent, chemiluminescent probes). This complex is used for many detection systems, such as immunoassays, DNA hybridization assays, immunohistochemistry and flow-cytometry. This approach is also applied for the purification and characterization of various molecules of interest.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis

Certificate of Origin

Milli-Q® Water Purification Solutions

The Emprove® Program
Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
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MSMLS Online Plate Map

Metabolite descriptors included with the software download upon purchase of the product include: Name, Parent CID, molecular formula, molecular weight, CAS, ChEBI, HMDB ID, PubChem Compound and Subst...
Keywords: Mass spectrometry, Metabolites

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