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P7949 Sigma-Aldrich

TWEEN® 20

BioXtra, viscous liquid

Synonym: Polyethylene glycol sorbitan monolaurate, Polyoxyethylenesorbitan monolaurate

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Properties

Related Categories Antigen-Vaccine Preparation, Biochemicals and Reagents, Cell Culture, Core Bioreagents, Detergents,
description   non-ionic
product line   BioXtra
form   viscous liquid
mol wt   mol wt ~1228
composition   lauric acid, ≥40% (balance primarily myristic, palmoyic, and stearic acids)
impurities   ≤0.05% Phosphorus (P)
ign. residue   ≤0.2%
refractive index   n20/D 1.468(lit.)
CMC   0.06 mM(20-25°C)
transition temp   cloud point 76 °C
density   1.095 g/mL at 25 °C(lit.)
anion traces   chloride (Cl-): ≤0.05%
  sulfate (SO42-): ≤0.05%
cation traces   Al: ≤0.0005%
  Ca: ≤0.0005%
  Cu: ≤0.0005%
  Fe: ≤0.0005%
  K: ≤0.005%
  Mg: ≤0.0005%
  NH4+: ≤0.05%
  Na: ≤0.05%
  Pb: ≤0.001%
  Zn: ≤0.0005%
HLB   16.7

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Application

Non-ionic detergent

TWEEN 20 is a nonionic detergent widely used in biochemical applications. It has been used as an emulsifying agent for the preparation of stable oil-in-water emulsions. TWEEN 20 has been used in pre-extraction of membranes to remove peripheral proteins (used at 2% for extraction of membrane-bound proteins).
TWEEN 20 has been used as a blocking agent for membrane based immunoassays at a typical concentration of 0.05%. TWEEN 20 can be used for lysing mammalian cells at a concentration of 0.005 to 0.5%.

Caution

TWEEN 20 may not be stable to autoclaving, particularly with metal cations in buffer solutions. Autoclaving is not recommended without testing for changes in properties. TWEEN 20 is heat sensitive and will darken when exposed to elevated temperatures. Polysorbates have been reported to be incompatible with alkalis, heavy metal salts, phenols, and tannic acid. They may also reduce the activity of many preservatives. Aqueous solutions of polysorbates undergo autoxidation during storage, with changes being catalyzed by light, increased temperature, and copper sulfate.

Packaging

100, 500 mL in poly bottle

Preparation Note

TWEEN 20 is miscible in water (100mg/mL), yielding a clear, yellow solution. It is also miscible with alcohol, dioxane, and ethyl acetate. TWEEN 20 is insoluble in liquid paraffin and fixed oils.

Legal Information

TWEEN is a registered trademark of Croda International PLC

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Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
1
RTECS 
TR7400000
Flash Point(F) 
>230 °F
Flash Point(C) 
>110 °C

Frequently Asked Questions

What is the percentage of Tween 20 in P7949?
P7949 is not diluted in any solvent. Thus it can be considered "100%" or the "neat" material.If you still need to ask a question, please click the Ask a Question tab above.
Can Product P7949, Tween® 20, be autoclaved?
Increased temperature can lead to autoxidation of Tween 20 in solution. Autoclaving is not recommended without testing for changes in properties. TWEEN 20 may not be stable to autoclaving, particularly with metal cations in buffer solutions. TWEEN 20 is heat sensitive; it will darken when exposed to elevated temperatures. Autoclaving is done at the customer's risk. Here is the link to the product information sheet: http://www.sigmaaldrich.com/etc/medialib/docs/Sigma-Aldrich/Product_Information_Sheet/p7949pis.Par.0001.File.tmp/p7949pis.pdf
Is Product P7949, Tween® 20, light-sensitive?
Light can catalyze autoxidation of Tween 20 during storage, so Tween 20 can be considered to be light sensitive. Here is the link to the product information sheet for this item: http://www.sigmaaldrich.com/etc/medialib/docs/Sigma-Aldrich/Product_Information_Sheet/p7949pis.Par.0001.File.tmp/p7949pis.pdf
What does "BioXtra" mean?
"BioXtra" is the grade designation given to Sigma-Aldrich products that have been tested be low in trace metal content.   For additional information, click on the following link: http://www.sigmaaldrich.com/etc/medialib/docs/Sigma-Aldrich/Datasheet/1/sigma-aldrich-grading-chart.Par.0001.File.tmp/sigma-aldrich-grading-chart.pdf
The appearance of Product P7949, Tween® 20, is not colorless. Is this OK?
The specification for P7949 is a clear yellow-green liquid.
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
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Peer-Reviewed Papers
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References

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