EMAIL THIS PAGE TO A FRIEND
01673 Sigma-Aldrich

Acriflavine

BioReagent, suitable for fluorescence, ≥95.0% (AT)

Synonym: 3,6-Diamino-10-methylacridinium chloride mixt. with 3,6-diaminoacridine (proflavine), Euflavine, Trypaflavine Neutral

  • CAS Number 8048-52-0

  • Empirical Formula (Hill Notation) C14H14ClN3

  • Molecular Weight 259.73

  •  MDL number MFCD00064307

  •  PubChem Substance ID 329747342

Purchase

Properties

Related Categories Alphabetical, Biochemicals and Reagents, Detection, Fluorescent Probes, Labels, Particles and Stains, Fluorescent Stains,
InChI Key   PEJLNXHANOHNSU-UHFFFAOYSA-N
product line   BioReagent
assay   ≥95.0% (AT)
composition   Sum of components 3,6-diaminoacridinium chloride and 3,6-diamino-10-methylacridinium chloride, ≥70% HPLC
solubility   H2O: 0.33 g/mL (lit.)(lit.)
fluorescence   λex 463 nm; λem 490 nm in ethanol
suitability   suitable for fluorescence

Description

Application

Acriflavine is used to fluorescently label high molecular weight RNA molecules .
Excitation and emission wavelengths in various solvents :
• Methanol: λex = 424 nm; λem = 518 nm
• Ethanol: λex = 426 nm; λem = 524 nm
• Propanol: λex = 430 nm; λem = 512 nm
• Butanol: λex = 430 nm; λem = 526 nm
• Formamide: λex = 434 nm; λem = 524 nm
• Glycerol: λex = 432 nm; λem = 540 nm
• Water: λex = 416 nm; λem = 514 nm

Insoluble in ether, chloroform, and fixed oils. Utilized in fluorescence steady state measurements as a donor molecule (when paired with rhodamine 6G as the acceptor) to function as a pH sensor .

Other Notes

DNA fluorochrome. Application in dual fluorescence analysis of cellular DNA and protein simultaneously

Safety & Documentation

Safety Information

Symbol 
Signal word 
Warning
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
RIDADR 
UN 3077 9 / PGIII
WGK Germany 
3
Protocols & Articles
Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?