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12133C Sigma-Aldrich

Bovine Calf Serum

USA origin, sterile-filtered, suitable for cell culture

Synonym: Calf Serum, bovine calf sera, calf sera, calf serum

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Properties

Related Categories Cell Culture, Fetal Bovine Serum (FBS) and Other Animal Sera, Newborn, Calf and Adult Bovine Sera More...
biological source   bovine (calf)
sterility   sterile-filtered
composition   hemoglobin, ≤35 mg/dL
origin   USA origin
application(s)   cell culture | mammalian: suitable
impurities   ≤10 EU/mL endotoxin
shipped in   dry ice
storage temp.   −20°C

Description

General description

Animal serum is commonly used to supplement basal media formulations for the optimal growth of many cell types in vitro. Bovine Calf Serum (BCS) is collected from calves that are less than 12 months old. The serum contains approximately double the protein of fetal bovine serum (FBS). BCS will promote growth in a variety of mammalian cell lines and is often used as a cost-effective substitute for FBS.

Application

Bovine Calf Serum has been used as a basal media supplement for growing :
• Immunocompetent cells of ileal Peyer’s patches
• Mouse skeletal myoblasts
• Ovarian cell lines
• Bovine retinal pericytes

Bovine Calf Serum has been used as a basal media supplement for growing:
• immunocompetent cells of ileal Peyer′s patches
• mouse skeletal myoblasts
• ovarian cell lines
• bovine retinal pericytes

Use in the propogation of viruses, Helicobacter pylori and Plasmodium falciparum.

Biochem/physiol Actions

Animal serum is commonly used to supplement basal media formulations for the optimal growth of many cell types in vitro. Bovine Calf Serum (BCS) is collected from calves that are less than 12 months old. The serum contains approximately double the protein of fetal bovine serum (FBS). BCS will promote growth in a variety of mammalian cell lines and is often used as a cost-effective substitute for FBS.

Testing Method

Endotoxin tested

Linkage

Request a serum sample

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Pipette Controller from Brand
Protocols & Articles

Articles

Serum for Cell and Tissue Culture: Frequently Asked Questions (FAQs)

Serum is added as a supplement to culture media at a concentration of 2-10% to provide a mixture of nutritional, hormonal, growth and attachment factors. Serum also acts as a buffer to the cell cultu...
Keywords: Agriculture, Cell culture, Centrifugation, Culture media, Degradations, Filtration, Nucleic acid denaturation

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Cell Culture Troubleshooting Guide

Cell culture has become one of the most fundamental techniques for modeling biological systems, and is of increasing importance in the biotechnology and pharmaceutical sectors as well as an essential...
Keywords: Antibiotics, Apoptosis, Cell attachment, Cell culture, Cryopreservation, Filtration, Growth factors, Pharmaceutical, Phase transitions

Peer-Reviewed Papers
15

References

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