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27813 Sigma-Aldrich

Coomassie protein assay reagent

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Properties

Related Categories Acids, Acids & Bases, Chemical Synthesis, Organic Acids, Synthetic Reagents More...
Quality Level   200
density   ~1.31 g/mL at 20 °C
storage temp.   2-8°C
SMILES string   [Na+].CCOc1ccc(Nc2ccc(cc2)C(\c3ccc(cc3C)N(CC)Cc4cccc(c4)S([O-])(=O)=O)=C5/C=CC(\C=C5C)=[N+](\CC)Cc6cccc(c6)S([O-])(=O)=O)cc1
InChI   1S/C47H49N3O7S2.Na/c1-6-49(31-35-11-9-13-43(29-35)58(51,52)53)40-21-25-45(33(4)27-40)47(37-15-17-38(18-16-37)48-39-19-23-42(24-20-39)57-8-3)46-26-22-41(28-34(46)5)50(7-2)32-36-12-10-14-44(30-36)59(54,55)56;/h9-30H,6-8,31-32H2,1-5H3,(H2,51,52,53,54,55,56);/q;+1/p-1
InChI key   RWVGQQGBQSJDQV-UHFFFAOYSA-M

Description

Packaging

250 mL in glass bottle

Application

Suitable for the determination of protein according to Bradford. Five-fold concentrate for tests in microtiter plates; ten-fold concentrate for tests in photometric cuvettes (d=1cm); dilute with H2O

Safety & Documentation

Safety Information

Signal word 
Danger
Target organs 
Eyes
RIDADR 
UN 2924 8(3) / PGII
WGK Germany 
WGK 2
Flash Point(F) 
109.4 °F
Flash Point(C) 
43 °C

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

Introduction to Western Blotting

The transfer of macromolecules such as nucleic acids and proteins to solid-phase membranous support is termed as blotting. Proteins resolved on Sodium dodecyl sulfate - polyacrylamide gel electrophor...
Keywords: Buffers, Cell disruption, Detergents, Electroblotting, Electrophoresis, Gel electrophoresis, Homogenization, Immobilization, PAGE, Protein assay, Protein extraction, Sample preparations, Separation, Size-exclusion chromatography, Western blot

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Peer-Reviewed Papers
15

References

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