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  • 3010-05A - Human Subclavian Artery Endothelial Cells: HScAEC, adult

3010-05A Sigma-Aldrich

Human Subclavian Artery Endothelial Cells: HScAEC, adult

  •  NACRES NA.81



Related Categories Cell Culture, Endothelial Cells, Primary Cell Culture, Primary Cells
Quality Level   100
biological source   human subclavian artery (normal)
packaging   pkg of 500,000 cells
growth mode   Adherent
karyotype   2n = 46
morphology   Endothelial
application(s)   cell culture | mammalian: suitable
relevant disease(s)   bipolar disorder, cancer, cardiovascular diseases
shipped in   dry ice
storage temp.   −196°C


General description

HScAEC, along with human aortic (HAOEC), carotid artery (HCtAEC), coronary artery (HCAEC) and brachiocephalic artery (HBcAEC) have been used to demonstrate that not only blood vessels from different tissues are highly heterogeneous, they also interact differently with leukocytes during the inflammation response (Scott, 2013). The authors further showed that differential N-glycosylation of commonly expressed vascular adhesion molecules may be responsible for this heterogeneity, as well as for modulation of signaling under resting and activated inflammatory conditions. This also explains why specific vascular beds may be more or less susceptible to particular diseases or stimuli. Importantly, if cells from different sources were used, these results could not be convincingly validated due to a number of uncontrolled variables, such as age, race, genetic variability or life style choices of the donors.

Because of the complex heterogeneity that exists not only between different donors, but even between different vascular beds in the same individual, it would be prudent to confirm any new findings on primary cell lots coming from several different origins.

Cell Line Origin



blood pressure, blood supply, tissue oxygenation


Basal Medium containing 10% FBS & 10% DMSO

Preparation Note

• 2nd passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
• Can be cultured at least 10 doublings

Subculture Routine

Please refer to the HScAEC Culture Protocol.

Safety & Documentation

Safety Information

NONH for all modes of transport


Certificate of Analysis (COA)

Please Enter a Lot Number

Pipette Controller from Brand
Protocols & Articles


Human Subclavian Artery Endothelial Cells (HScAEC) Culture Protocol

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