• USA Home
  • 408-05A - Human Fibroblast-Like Synoviocytes: HFLS, adult

EMAIL THIS PAGE TO A FRIEND
408-05A Sigma-Aldrich

Human Fibroblast-Like Synoviocytes: HFLS, adult

  •  NACRES NA.81

Purchase

Properties

Related Categories Cell Culture, Primary Cell Culture, Primary Cells, Synoviocytes
Quality Level   100
biological source   human synovial tissues (normal)
packaging   pkg of 500,000 cells
growth mode   Adherent
karyotype   2n = 46
morphology   Fibroblast-like
application(s)   cell culture | mammalian: suitable
relevant disease(s)   arthritis
shipped in   dry ice
storage temp.   −196°C

Description

General description

Normal HFLS provide an excellent cellular model for studying the normal and pathological physiology of synoviocytes and development of joint diseases.

Normal HFLS have been used in numerous research studies to:

• Study changes in gene expression in synoviocytes stimulated with TNFα (Suggiyama, 2002)
• Demonstrate that TNF-like weak inducer of apoptosis (TWEAK) contributes to joint inflammation by inducing chemokines such as MIP-1β (CCL-4), lymphotactin (XCL-1), IFN-γ-inducible protein 10 (IP-10) (CXCL-10), MCP-1 (CCL-2), and RANTES (CCL-5), as well as the matrix metalloprotease-9, suggesting TWEAK as a new therapeutic target (Perper, 2006)
• Demonstrate the role of miR-124a in arthritis pathogenesis (Nakamachi, 2009)
• Show that C/EBPβ regulates expression of metalloproteinases and ADAMTS family members in synoviocytes stimulated with IL-1β (Tsushima, 2012); and that HMW-HA suppressed ADAMTS4 mRNA and protein expression via CD44, p38 MAPK and JNK pathways (Kataoka, 2013)
• Evaluate the anti-inflammatory and antirheumatic activity of various compounds, such as NF-κB inhibitors (Wen, 2006), AGIX-4207 (Kunsch, 2005), HA–methotrexate conjugates (Homma, 2009) and bucillamine (Oki, 2009)
• Show that reactive arthritis triggered by chlamydial infection is mediated by TLR2 induced IL-6 production in synoviocytes (Konomi, 2009); and investigate the mechanisms of arthritis-like syndrome in patients infected with chikungunya (CHIK) virus (Xu, 2013)
• Suggest that only leukocyte-poor, RBC-free platelet-rich plasma should be used in clinical orthopaedics because leukocytes and RBCs cause synoviocyte death and proinflammatory mediator production (Brown, 2014)
• Identify that cartilage link protein and MAGP2 can be used as specific markers to distinguish chondrocytes and synovial cells (Rapko, 2007, 2010)
• Create, along with human dermal fibroblasts, induced pluripotent stem cells (iPSC) by using the now classic “Yamanaka cocktail”, the discovery for which Dr. Shinya Yamanaka was awarded the Nobel Prize in 2012 (Takahashi, 2007)

Additionally, in parallel with HFLS isolated from joints of patients with rheumatoid arthritis, normal HFLS were used to:

• Identify causing agents (such as uric acid crystals or platelet microparticles) and study the immunopathological mechanisms and signal transduction pathways leading to joint inflammation in rheumatoid arthritis (Chen, 2011a; Hsu, 2012; Mathieu, 2008; Tsuji, 2012), and to demonstrate the role of estrogen signaling in increasing inflammation (Galal, 2008)
• Investigate anti-inflammatory properties of herbal compound Sinomenine suggested for rheumatoid arthritis treatment (Chen, 2011b)
• Study the effects of extracellular matrix composition on cell attachment and migration relevant to T-cell function in inflamed tissues (Evanko, 2012)

Finally, all three types of HFLS (normal, RA and OA) were used to investigate the role of human endogenous retroviruses (HERVs) in development of rheumatoid arthritis, and suggest that activated expression of different forms of HERV contribute to development of rheumatoid arthritis symptoms by different mechanisms (Freimanis, 2010).

Cell Line Origin

Bone

Application

synoviocyte physiology, joint homeostasis, production of collagen, glycoproteins, lubrican, vimentin and hyaluronic acid, gene expression, signal transduction pathways, enzyme production, evaluation of anti-inflammatory agents, effects of extracellular matrix, cell attachment, migration, integrin study, proinflammatory mediator production

Components

Basal Medium containing 10% FBS & 10% DMSO

Preparation Note

• 2nd passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
• Can be cultured at least 5 doublings

Subculture Routine

Please refer to the HFLS Culture Protocol.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number

Pipette Controller from Brand
Protocols & Articles

Protocols

Human Fibroblast-Like Synoviocytes (HFLS) Culture Protocol

*Be sure to wear face protection mask and gloves when retrieving cryovials from the liquid nitrogen storage tank. The dramatic temperature change from the tank to the room could cause any trapped liq...
Keywords: Cell culture, Sterilizations

Related Content

Cell Culture Troubleshooting Guide

Cell culture has become one of the most fundamental techniques for modeling biological systems, and is of increasing importance in the biotechnology and pharmaceutical sectors as well as an essential...
Keywords: Antibiotics, Apoptosis, Cell attachment, Cell culture, Cryopreservation, Filtration, Growth factors, Pharmaceutical, Phase transitions

Introduction to Primary Cell Culture [VIDEO]

Interested in receiving more content like this? Join over 8,000 of your peers who have already signed up for the Cell Informer newsletter.
Keywords: Cell culture, Drug discovery, Pharmaceutical

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?