• USA Home
  • 62335 - Lipoprotein Lipase from Pseudomonas sp.

62335 Sigma-Aldrich

Lipoprotein Lipase from Pseudomonas sp.

lyophilized, powder, ≥1200 U/mg



General description

Lipoprotein Lipase (LPL) is majorly secreted by myocytes and adipocytes in humans and is crucial for triglyceride homeostatis. Mutations in the catalytic domain of LPL impairs its interaction with glycosylphosphatidylinositol anchored high density lipoprotein binding protein 1 (GPIHBP1). The N-terminal catalytic domain is essential for lipolysis. The C-terminal is crucial for binding lipoproteins. Altered LPL levels may play role in the pathogenesis of atherosclerosis, coronary heart disease and chronic lymphocytic leukemia.


Lipoprotein Lipase from Pseudomonas sp. has been used in the enzymatic erosion studies in gamma irradiated poly(trimethylene carbonate) (PTMC) films.

Biochem/physiol Actions

Malic dehydrogenase catalyzes the dehydrogenation of L-malate by NAD+.

Lipoprotein lipase belongs to the family of triglyceride lipases. It hydrolyses triglycerides in triglyceride-rich ApoB-containing lipoproteins.

Unit Definition

1 U corresponds to the amount of enzyme which liberates 1 μmol oleic acid per minute at pH 8.0 and 40°C (triolein, Cat. No. 62314 as substrate)

Other Notes

Preparation of aldol acceptors (R)- and (S)-3-azido-2-hydroxypropanal via lipase-catalyzed resolution of the racemic acetal precursor; Effect of enzyme form on its properties in toluene

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles


Roar Assay Kits for Studies in Lipoprotein Metabolism

This technical article is a compilation of select fluorescence-based activity assays developed by Roar Biomedical now offered through Sigma-Aldrich in kit format. The methods are well-characterized, ...
Robert Brocia
Roar Biomedical, Inc.
Keywords: Addition reactions, Cardiovascular, Chromatography, Clinical, Detection methods, Drug discovery, Esterifications, Gas chromatography, High performance liquid chromatography, Separation, Thin layer chromatography


LPL Activity Assay Protocol

The kit is sufficient for 100 assays in 200 µL total assay volume. Substrate Emulsion                                    0.125 mL Catalog Number MAK109A LPL Standard, 75.7 mM                         ...
Keywords: Sample preparations

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers


Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?