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63301-001 Sigma-Aldrich

PCRboost®

Trial Kit, for improved PCR performance

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Properties

Related Categories Biochemicals and Reagents, Biostabilization Agents, Stabilization of Biomolecules More...
form   liquid
usage   3 reactions
storage temp.   room temp

Description

General description

PCRboost® is a unique reagent that enhances end-point and reverse transcription-PCR performance by improving sensitivity and specificity during amplification of genomic DNA or RNA templates. To use, simply replace the water in your PCR with PCRboost®. That′s all. It is designed to enhance the amplification of DNA without the need for time consuming optimizations. PCRboost® successfully amplifies thermally degraded DNA resulting in a visible amplicon that can be used for downstream applications such as cloning and sequencing.

Don’t see what you are looking for? Custom stabilization services for molecular diagnostic assay and reagent manufacturers are available through Sigma′s partner, Biomatrica. PCRstable® enables the preservation of PCR-based assay components in any format (liquid or dry).

Packaging

1 bottle

Physical form

150 μL of PCRboost® in 1.5 mL screw-top conical tube

Legal Information

PCRboost is a registered trademark of Biomatrica

PCRstable is a registered trademark of Biomatrica

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

PCRboost™: A Novel PCR Amplification Enhancer

Polymerase chain reaction (PCR) is one of the most performed molecular techniques not only in the academic and industrial research world but also in the forensics, veterinary and health industries. I...
Keywords: Amplification, Growth factors, Industries, Polymerase chain reaction, Reductions, Size-exclusion chromatography, Veterinary

Successful Amplification of Thermally Degraded DNA Using PCRboost™

DNA samples are often exposed to harsh environmental conditions, including heat, humidity, UV or chemicals. Some procedures, such as the extraction of samples from formalin-fixed paraffin embedded ti...
Keywords: AGE, Amplification, Cloning, Degradations, Electrophoresis, Environmental, Gas chromatography, Gel electrophoresis, Polymerase chain reaction, Sequencing, Size-exclusion chromatography

Protocols

PCRboost™ Protocol for enhanced DNA amplification

Polymerase chain reaction (PCR) is one of the most performed molecular techniques not only in the academic and industrial research world but also in the forensics, veterinary and health industries. I...
Keywords: Amplification, Industries, Polymerase chain reaction, Size-exclusion chromatography, Veterinary

Related Content

Greener Alternatives Four Categories

A new icon has been added to identify our Greener alternative products. Products with this icon fulfill one of the four criteria.
Keywords: Alternative energy, Environmental, Green chemistry, PAGE, Solvents, Substitutions

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63301-011 PCRboost®, 1 mL bottle, for improved PCR performance

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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