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72172 Sigma-Aldrich

Fluorescent IEF-Marker pI 3.0 solution

Synonym: IEF-Marker fluorescent pI 3.0

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Properties

Related Categories Analytical Standards, Analytical/Chromatography, Capillary Electrophoresis, Capillary Isoelectric Focusing, Electrophoresis,
Quality Level   100
mol wt   Mw 215.63 g/mol
concentration   2 mg/mL in 5 mM HCl, 50% 2-propanol
fluorescence   λex 360 nm; λem 445 nm in 0.1 M citrate pH 3.0
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storage temp.   2-8°C

Description

General description

filtered through a 0.45 μm membrane filter

Application

Suitable as IEF-Marker for HPCE and for IEF-gels

Reconstitution

For use in HPCE the marker should be diluted 1:100 in a suitable ampholyte solution (e.g. 2% ampholyte 3-10), UV-detection at 280 nm (20 °C). For use in IEF-gels the solution can be loaded directly onto the gel (max. 1μl per lane).

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Precautionary statements 
Target organs 
Central nervous system
RIDADR 
UN 1987C 3 / PGIII
WGK Germany 
WGK 1
Protocols & Articles

Articles

Isoelectric Focusing (IEF)

Isoelectric focusing (IEF) is a powerful analytical tool for the separation of proteins. In order to ensure the high performance of analysis, isoelectric point (pI) standards are needed. In addition ...
Monika Baeumle
BioFiles 2009, 4.1, 14.
Keywords: Absorption, Capillary electrophoresis, Electrophoresis, Gel electrophoresis, Immobilization, Isoelectric focusing, Separation

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