A1720 Sigma-Aldrich

G 418 disulfate salt

powder, BioReagent, suitable for cell culture

Synonym: Antibiotic G418

  • CAS Number 108321-42-2

  • Empirical Formula (Hill Notation) C20H40N4O10 · 2H2SO4

  • Molecular Weight 692.71

  •  MDL number MFCD00058314

  •  PubChem Substance ID 24890581

  •  NACRES NA.76



Related Categories A - K, Aminoglycosides, Antibacterial, Antibiotics, Antibiotics A to Z,
product line   BioReagent
form   powder
optical activity   [α]/D (Specific Rotation: +104.4o (c=0.3% in H2O at 26oC))
potency   ≥450 μg per mg (Dried basis)
mol wt   692.7
packaging   pkg of 1 g
  pkg of 25 g
  pkg of 5 g
application(s)   cell culture | mammalian: suitable
color   white to off-white
solubility   H2O: 100 mg/mL (As a stock solution. Stock solutions should be stored at 2-8°C. Stable at 37°C for 8 days.)
Mode of action   protein synthesis | interferes
antibiotic activity spectrum   Gram-negative bacteria
storage temp.   2-8°C
SMILES string   OS(O)(=O)=O.OS(O)(=O)=O.CN[C@H]1[C@H](O)[C@@H](OC[C@@]1(C)O)O[C@H]2[C@H](N)C[C@H](N)[C@@H](O[C@H]3O[C@H]([C@H](C)O)[C@@H](O)[C@H](O)[C@H]3N)[C@@H]2O
InChI   1S/C20H40N4O10.2H2O4S/c1-6(25)14-11(27)10(26)9(23)18(32-14)33-15-7(21)4-8(22)16(12(15)28)34-19-13(29)17(24-3)20(2,30)5-31-19;2*1-5(2,3)4/h6-19,24-30H,4-5,21-23H2,1-3H3;2*(H2,1,2,3,4)/t6?,7-,8+,9+,10+,11-,12-,13-,14+,15+,16-,17-,18+,19-,20+;;/m0../s1


General description

Chemical structure: aminoglycoside

G-418 is an aminoglycoside antibiotic, which is produced by Micromonospora rhodorangea NRRL 5326 strain. It has a broad range of antibacterial activity both in vivo and in vitro. It also shows activity against protozoa, cecal amoebiasis, and helminthes. It is commonly known as geneticin selective antibiotic.


G418 disulfate salt is an aminoglycoside antibiotic similar in structure to gentamicin used for selection and maintenance of prokaryotic and eukaryotic cells transfected with a iNOS promoter construct and neomycin resistance gene†. Optimal concentrations for selection and maintenance vary by cell lines. Bacteria and algae require 5 μg/mL or less while animal cells may require 300-500 μg/mL.


1, 5, 25 g in glass bottle

Biochem/physiol Actions

G-418 inhibits protein synthesis at the early stages of elongation, post-initiation, at the initiation stage of translation as well as just before the initiation stage of translation.


The product is stable for two years when stored at 2-8°C. Stock solutions are stable at 37°C for 8 days.

Preparation Note

G418 powder is soluble in water at 100 mg/mL.

Other Notes

Keep container tightly closed in a dry and well-ventilated place. Keep in a dry place.

Safety & Documentation

Safety Information

GHS08  GHS08
Signal word 
Hazard statements 
Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 
Protocols & Articles


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