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A1720 Sigma

G 418 disulfate salt

powder, BioReagent, suitable for cell culture

Synonym: Antibiotic G418

  • CAS Number 108321-42-2

  • Empirical Formula (Hill Notation) C20H40N4O10 · 2H2SO4

  • Molecular Weight 692.71

  •  MDL number MFCD00058314

  •  PubChem Substance ID 24890581

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Properties

Related Categories A - K, Aminoglycosides, Antibacterial, Antibiotic powders, Antibiotics,
InChI Key   UHEPSJJJMTWUCP-NKCAIAFTSA-N
product line   BioReagent
form   powder
optical activity   [α]/D (Specific Rotation: +104.4o (c=0.3% in H2O at 26oC))
potency   ≥450 μg per mg (Dried basis)
mol wt   mol wt 692.7
packaging   pkg of 1 g
  pkg of 25 g
  pkg of 5 g
color   white to off-white
solubility   H2O: 50 mg/mL (As a stock solution. Stock solutions should be stored at 2-8°C. Stable at 37°C for 8 days.)
suitability   suitable for cell culture
Mode of action   protein synthesis | interferes
storage temp.   2-8°C

Description

Caution

The product is stable for three years when stored at 2-8°C. Stock solutions are stable at 37°C for 8 days.

Packaging

1, 5, 25 g in glass bottle

Preparation Note

G418 powder is soluble in water at 50 mg/mL.

Application

G418 disulfate salt is an aminoglycoside antibiotic similar in structure to gentamicin used for selection and maintenance of prokaryotic and eukaryotic cells transfected with a iNOS promoter construct and neomycin resistance gene†. Optimal concentrations for selection and maintenance vary by cell lines. Bacteria and algae require 5 μg/mL or less while animal cells may require 300-500 μg/mL.

Biochem/physiol Actions

G-418 inhibits protein synthesis at the early stages of elongation, post-initiation, at the initiation stage of translation as well as just before the initiation stage of translation.

General description

Chemical structure: aminoglycoside

G-418 is an aminoglycoside antibiotic, which is produced by Micromonospora rhodorangea NRRL 5326 strain. It has a broad range of antibacterial activity both in vivo and in vitro. It also shows activity against protozoa, cecal amoebiasis, and helminthes. It is commonly known as geneticin selective antibiotic.

Other Notes

Keep container tightly closed in a dry and well-ventilated place. Keep in a dry place.

Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Cell Culture Guide

Wired Weeks Savings
Protocols & Articles

Articles

Antibiotic Kill Curve

Antibiotic kill curve is a dose response experiment in which mammalian cells are subjected to increasing amounts of selection antibiotic to determine the minimum concentration of an antibiotic that c...
Keywords: Antibiotics, Cell culture, Evaporation, Transfection

Protocols

Correlative microscopy and electron tomography of GFP through photooxidation

Markus Grabenbauer a , Willie J C Geerts b , Julia Fernadez-Rodriguez c , Andreas Hoenger a , Abraham J Koster b , Tommy Nilsson c
Keywords: Apoptosis, Biochemistry, Catalysis, Catalytic combustion detector, Cell biology, Cell culture, Chromatin immunoprecipitation, Gene expression, Glycosylations, Microscopy, O-linked glycosylation, Oxidations, Polymerization reactions, Precipitation, Respiration, Scanning electron microscopy, Transfection, Transmission electron microscopy

High-efficiency yeast transformation using the LiAc/SS carrier DNA/PEG method

b Department of Pathology, Environmental Health and Radiation Oncology, UCLA School of Public Health and David Geffen School of Medicine
Keywords: Antibiotics, Biochemistry, Cell biology, Cell culture, Centrifugation, Environmental, Enzymology, Evaporation, Gene expression, Genetic, Genetics, Ion-exchange chromatography, Oncology, Sterilizations, transformation

Related Content

Antibiotics Selection Guide

The Antibiotic Selection Guide enables researchers to spend more time on their research discoveries and less time seeking established antibiotic information. Link to hundreds of antibiotics for conta...
Keywords: Antibiotics, Cell biology, Cell culture, Genetic, High performance liquid chromatography, Reversed-phase chromatography

Cell Culture Troubleshooting Guide

Cell culture has become one of the most fundamental techniques for modeling biological systems, and is of increasing importance in the biotechnology and pharmaceutical sectors as well as an essential...
Keywords: Antibiotics, Apoptosis, Cell attachment, Cell culture, Cryopreservation, Filtration, Growth factors, Pharmaceutical, Phase transitions

Mouse Embryonic Stem Cell Culture Procedures & Protocols

The development of transgenic and gene knockout technology has provided an effective tool for the analysis of gene function. Critical to this has been the ability to isolate and culture murine embryo...
Keywords: Antibiotics, Apoptosis, Cell culture, Culture media, PAGE

Peer-Reviewed Papers
15

References

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