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A2792 Sigma-Aldrich

Acrylamide/Bis-acrylamide

BioReagent, for molecular biology, 29:1 (ratio)

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Properties

Related Categories Acrylamide, Acrylamides, Molecular Biology, Nucleic Acid Electrophoresis, Protein Electrophoresis,
Quality Level   200
grade   for molecular biology
product line   BioReagent
feed ratio   29:1 (ratio)
foreign activity   DNase, RNase and protease, none detected
SMILES string   NC(=O)C=C.C=CC(=O)NCNC(=O)C=C
InChI   1S/C7H10N2O2.C3H5NO/c1-3-6(10)8-5-9-7(11)4-2;1-2-3(4)5/h3-4H,1-2,5H2,(H,8,10)(H,9,11);2H,1H2,(H2,4,5)
InChI key   OCQZXMCGTAWGEQ-UHFFFAOYSA-N

Description

General description

Acrylamide (AA) is a neurotoxin and carcinogen. It was found in potato products like, french fries and potato chips and also in several cereal-based products. This highly reactive organic compound has the ability to polymerize to form polyacrylamide.

Application

Acrylamide/Bis-acrylamide has been used in the preparation of polyacrylamide (PA) gels. It has also been used as a component in the preparation of blotting gel precursor solution, separation gel precursor solution and loading gel precursor solution.

Ready to reconstitute dry powder blends are accurately pre-blended to produce a 40% (w/v) stock solution for use in protein and nucleic acid electrophoresis. The concentration is based on the total weight of both the acrylamide and bis-acrylamide.

Packaging

1 L in poly bottle

100 mL in poly bottle

Features and Benefits

Eliminates the need to weigh toxic acrylamide and bis-acrylamide.

Reconstitution

Reconstitute by slowly adding the indicated amount of distilled water to the container and dissolve contents.

To make up 100 ml of A2792, slowly add 63 ml distilled or deionized water to the container and dissolve contents. Do not divide the solid. This makes 100 ml of a 40% stock solution.
For the 1 Liter size, use 630 mL of water.

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Target organs 
Peripheral nervous system
RIDADR 
UN 2074 6.1 / PGIII
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Buffers - From Bnech to Bulk
Protocols & Articles
Peer-Reviewed Papers
15

References

Related Products

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Product #

Description

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A7802 Acrylamide/Bis-acrylamide, 40% solution, BioReagent, suitable for electrophoresis, 29:1
A3574 Acrylamide/Bis-acrylamide, 30% solution, BioReagent, suitable for electrophoresis, 29:1

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