• USA Home
  • A2942 - Amphotericin B solution

EMAIL THIS PAGE TO A FRIEND
A2942 Sigma-Aldrich

Amphotericin B solution

250 μg/mL in deionized water, 0.1 μm filtered, BioReagent, suitable for cell culture

  • CAS Number 1397-89-3

  • Empirical Formula (Hill Notation) C47H73NO17

  • Molecular Weight 924.08

  •  MDL number MFCD00877763

  •  PubChem Substance ID 24890711

  •  NACRES NA.76

Purchase

Properties

Related Categories Antibiotic Solutions, Antibiotics, Antibiotics A to Z, Antibiotics by Mechanism of Action, Antibiotics  A-F,
Quality Level   200
sterility   0.1 μm filtered
product line   BioReagent
form   liquid
concentration   250 μg/mL in deionized water
application(s)   cell culture | mammalian: suitable
color   yellow
Mode of action   cell membrane | interferes
antibiotic activity spectrum   fungi
  yeast
shipped in   dry ice
storage temp.   −20°C
SMILES string   [H][C@]12C[C@@H](O[C@@H]3O[C@H](C)[C@@H](O)[C@H](N)[C@@H]3O)\C=C\C=C\C=C\C=C\C=C\C=C\C=C\[C@H](C)[C@@H](O)[C@@H](C)[C@H](C)OC(=O)C[C@H](O)C[C@H](O)CC[C@@H](O)[C@H](O)C[C@H](O)C[C@](O)(C[C@H](O)[C@H]1C(=O)O[Na])O2
InChI   1S/C47H73NO17/c1-27-17-15-13-11-9-7-5-6-8-10-12-14-16-18-34(64-46-44(58)41(48)43(57)30(4)63-46)24-38-40(45(59)60)37(54)26-47(61,65-38)25-33(51)22-36(53)35(52)20-19-31(49)21-32(50)23-39(55)62-29(3)28(2)42(27)56/h5-18,27-38,40-44,46,49-54,56-58,61H,19-26,48H2,1-4H3,(H,59,60)/b6-5+,9-7+,10-8+,13-11+,14-12+,17-15+,18-16+/t27-,28-,29-,30+,31+,32+,33-,34-,35+,36+,37-,38-,40+,41-,42+,43+,44-,46-,47+/m0/s1
InChI key   APKFDSVGJQXUKY-INPOYWNPSA-N

Description

General description

Amphotericin B is a polyene antifungal antibiotic that is clinically used to treat systemic fungal infections.

Chemical structure: polyene

Application

Amphotericin B is a polyene antifungal antibiotic from Streptomyce sp. It has a high affinity for sterols, especially ergosterols, of both fungal and bacterial cell membranes. Normal usage in maintenance of antifungal cell cultures is 2.5 mg/L with penicillin and streptomycin used in the medium.

Biochem/physiol Actions

Amphotericin B solution has been used-
• as an intraperitoneal injection for the treatment of Aspergillus fumigatus infection in mice
• as a constituent of aspiration medium for the culture of Chinese Merino ewe ovaries
• as a constituent of transport medium during the collection of equine corpora lutea
• as a supplement in RPMI medium for the culture of the mouse-mouse B cell hybridoma 55-6 cell line

Packaging

20,50,100ml

Components

This product is supplied as 250 μg/mL Amphotericin B in deionized water. It is a solution containing Amphotericin B, and the following components aimed to improve its solubility: sodium deoxycholate, sodium chloride and sodium phosphate.

Caution

Amphotericin B remains active for 3 days in culture at 37°C.

Preparation Note

It is not unusual for this material to form a precipitate in aqueous solutions. Mixing the solution will redissolve most of the precipitate but, if it does not fully resolubilize, the Amphotericin B will still be suitable for use.

Other Notes

Keep container tightly closed in a dry and well-ventilated place. Light sensitive.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How should Product A2942, Amphotericin B solution, be stored?
This product should be stored at -20 °C.It is stable in culture at 37 °C up to 3 days.
At what concentration should Product A2942, Amphotericin B solution, be used?
We suggest a working concentration of 10 ml/L (2.5 mg/L).
What is the shelf life of Product A2942, Amphotericin B solution?
It has an expiration date of 18 months. This can be found on the lot-specific certificate of analysis.
How do I find price and availability?
There are several ways to find pricing and availability for our products.Once you log onto our website, you will find the price and availability displayed on the product detail page.You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 orview local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
Show more questions

Single- & multi-channel pipettes from BRAND

Pipette Controller from Brand
Protocols & Articles

Articles

Antibiotic Kill Curve

Antibiotic kill curve is a dose response experiment in which mammalian cells are subjected to increasing amounts of selection antibiotic to determine the minimum concentration of an antibiotic that c...
Keywords: Antibiotics, Cell culture, Evaporation, Transfection

Human Microbiome Research Workflow: An Overview of the Role in Human Health and Disease

Previously, the study of human microbiology was based on identification, isolation and culture of single microbes, such as bacteria, fungi and viruses. Scientists collected these organisms from patie...
Keywords: Amplification, Antibiotics, Antifungals, Cell culture, Cell disruption, Clinical, Diabetes, Digestions, Diseases, Environmental, Gas chromatography, Gas chromatography mass spectrometry, Gastrointestinal, Genetics, Genomics, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolites, Metabolomics, Microbiology, Nutrition, Obesity, Polymerization reactions, Proteomics, Respiratory, Sample preparations, Separation, Sequencing

Human Renal Proximal Tubule Epithelial Cells (RPTEC) as an Essential Advanced Model to Express Kidney Drug Transporters & Toxicity

Human renal proximal tubule epithelial cells (RPTECs) are commonly used to predict human renal drug permeability and to investigate drug efflux. However, current methods for investigating drug-transp...
Keywords: Cell culture, Chromatin immunoprecipitation, Gene expression, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Polymerase chain reaction

Related Content

Antibiotics Selection Guide

The Antibiotic Selection Guide enables researchers to spend more time on their research discoveries and less time seeking established antibiotic information. Link to hundreds of antibiotics for conta...
Keywords: Antibiotics, Cell biology, Cell culture, Genetic, High performance liquid chromatography, Reversed-phase chromatography

Cell Culture Troubleshooting Guide

Cell culture has become one of the most fundamental techniques for modeling biological systems, and is of increasing importance in the biotechnology and pharmaceutical sectors as well as an essential...
Keywords: Antibiotics, Apoptosis, Cell attachment, Cell culture, Cryopreservation, Filtration, Growth factors, Pharmaceutical, Phase transitions

Peer-Reviewed Papers
15

References

Related Products

related product

Product #

Description

Add to Cart

92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?