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A2942 Sigma-Aldrich

Amphotericin B solution

250 μg/mL in deionized water, 0.1 μm filtered, BioReagent, suitable for cell culture

  • CAS Number 1397-89-3

  • Empirical Formula (Hill Notation) C47H73NO17

  • Molecular Weight 924.08

  •  MDL number MFCD00877763

  •  PubChem Substance ID 24890711

  •  NACRES NA.76



Related Categories Antibiotic Solutions, Antibiotics, Antibiotics A to Z, Antibiotics by Mechanism of Action, Antibiotics  A-F,
Quality Level   200
sterility   0.1 μm filtered
product line   BioReagent
form   liquid
concentration   250 μg/mL in deionized water
application(s)   cell culture | mammalian: suitable
color   yellow
Mode of action   cell membrane | interferes
antibiotic activity spectrum   fungi
shipped in   dry ice
storage temp.   −20°C
SMILES string   [H][C@]12C[C@@H](O[C@@H]3O[C@H](C)[C@@H](O)[C@H](N)[C@@H]3O)\C=C\C=C\C=C\C=C\C=C\C=C\C=C\[C@H](C)[C@@H](O)[C@@H](C)[C@H](C)OC(=O)C[C@H](O)C[C@H](O)CC[C@@H](O)[C@H](O)C[C@H](O)C[C@](O)(C[C@H](O)[C@H]1C(=O)O[Na])O2
InChI   1S/C47H73NO17/c1-27-17-15-13-11-9-7-5-6-8-10-12-14-16-18-34(64-46-44(58)41(48)43(57)30(4)63-46)24-38-40(45(59)60)37(54)26-47(61,65-38)25-33(51)22-36(53)35(52)20-19-31(49)21-32(50)23-39(55)62-29(3)28(2)42(27)56/h5-18,27-38,40-44,46,49-54,56-58,61H,19-26,48H2,1-4H3,(H,59,60)/b6-5+,9-7+,10-8+,13-11+,14-12+,17-15+,18-16+/t27-,28-,29-,30+,31+,32+,33-,34-,35+,36+,37-,38-,40+,41-,42+,43+,44-,46-,47+/m0/s1


General description

Amphotericin B is a macrocyclic lactone with a hydrophilic polyhydroxyl chain and a lipophilic polyene based hydrocarbon chain.


Amphotericin B solution has been used:
• as an intraperitoneal injection for the treatment of Aspergillus fumigatus infection in mice
• as a constituent of aspiration medium for the culture of Chinese Merino ewe ovaries
• as a constituent of transport medium during the collection of equine corpora lutea
• as a supplement in RPMI medium for the culture of the mouse-mouse B cell hybridoma 55-6 cell line

Biochem/physiol Actions

Amphotericin B is a polyene antifungal antibiotic from Streptomyces sp. that is clinically used to treat systemic fungal infections. It has a high affinity for sterols, especially ergosterols, of both fungal and bacterial cell membranes. Normal usage in maintenance of antifungal cell cultures is 2.5 mg/L with penicillin and streptomycin used in the medium.

Amphotericin B is most effective against fungi and yeast. It forms transmembrane channels by binding to sterols associated with membranes, thereby leading to the leakage of small molecules. Amphotericin B elicits toxicity despite being an effective antibiotic for treating mycoses.




This product is supplied as 250 μg/mL Amphotericin B in deionized water. It is a solution containing Amphotericin B, and the following components aimed to improve its solubility: sodium deoxycholate, sodium chloride and sodium phosphate.


Amphotericin B remains active for 3 days in culture at 37°C.

Preparation Note

It is not unusual for this material to form a precipitate in aqueous solutions. Mixing the solution will redissolve most of the precipitate but, if it does not fully resolubilize, the Amphotericin B will still be suitable for use.

Other Notes

Keep container tightly closed in a dry and well-ventilated place. Light sensitive.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How should Product A2942, Amphotericin B solution, be stored?
This product should be stored at -20 °C.It is stable in culture at 37 °C up to 3 days.
At what concentration should Product A2942, Amphotericin B solution, be used?
We suggest a working concentration of 10 ml/L (2.5 mg/L).
What is the shelf life of Product A2942, Amphotericin B solution?
It has an expiration date of 18 months. This can be found on the lot-specific certificate of analysis.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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