A3151 Sigma-Aldrich


lyophilized powder

Synonym: Avidin-POD



Related Categories Antibodies, Antibody Conjugates, Avidin Reagents, Biotin/Avidin, Molecular Biology,
Quality Level   200
biological source   avidin from egg white
  enzyme from horseradish
form   lyophilized powder
composition   Protein, ≥70% E1%/280
extent of labeling   0.7-2.0 mol peroxidase per mol avidin
application(s)   direct ELISA: 1:50,000
solubility   H2O: soluble 1 mg/mL
  PBS: soluble
conjugate   peroxidase conjugate
storage temp.   −20°C


General description

Avidin is homotetrameric protein (68kDa) that is obtained from egg whites. This egg protein binds strongly to biotin. Thus, avidin-biotin association has been utilized in immunoassays to detect the localization of antigens in tissues. The use of avidin-biotin immunoassay enhances the sensitivity of the technique and facilitates the detection of antigens in low quantities.


Avidin-Peroxidase has been used for detecting anti-5-bromodeoxyuridine (anti-BrdU) binding and OVA-IgG1.

To detect Ga1-deficient IgA1 antibodies in mouse serum, ELISA assays were performed using sera which was incubated with biotinylated HAA, which binds IgA1, in 96-well plates. HRP-conjugated avidin was then incubated with the sera for 40 minutes at 37 degrees.

Physical form

Lyophilized powder containing citrate buffer.

Preparation Note

Labeled with Type VI peroxidase. Coupled by a modification of the method of Sullivan, M.J., et al., FEBS Lett., 95, 311 (1978).


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles


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92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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