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A9469 Sigma-Aldrich

Monoclonal ANTI-FLAG® M2-Alkaline Phosphatase antibody produced in mouse

clone M2, purified immunoglobulin, buffered aqueous glycerol solution

Synonym: Anti-ddddk, Anti-dykddddk, Monoclonal ANTI-FLAG® M2 antibody produced in mouse

  •  NACRES NA.32

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Properties

Related Categories Alkaline Phosphatase Conjugates, Alphabetical Index, Antibodies, Antibodies and Conjugates, Antibodies to Peptide and Protein Fusion Tags,
conjugate   alkaline phosphatase conjugate
clone   M2, monoclonal
biological source   mouse
application(s)   indirect ELISA: 1:20,000
species reactivity   all
form   buffered aqueous glycerol solution
shipped in   wet ice
storage temp.   −20°C
antibody form   purified immunoglobulin
isotype   IgG1
Quality Level   200
antibody product type   primary antibodies
concentration   ~1 mg/mL
immunogen sequence   DYKDDDDK

Description

General description

Monoclonal ANTI-FLAG® M2-Alkaline Phosphatase is a purified IgG 1 mouse antibody covalently conjugated to calf intestinal alkaline phosphatase (AP). The antibody conjugate binds to FLAG® fusion proteins and will recognize the FLAG® epitope at any position in the fusion protein (N-terminal, Met-N-terminal, C-terminal or internal FLAG® peptides).

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Monoclonal ANTI-FLAG® M2-Alkaline Phosphatase antibody produced in mouse has been used:
• in direct tissue blot immunoassay of sweet orange petioles samples
• in screening internalization of delta opioid receptor
• for screening cell-free protein expression using ELISA

Physical form

Solution in Tris buffered saline containing 50% glycerol plus stabilizer and preservative

Legal Information

ANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC

FLAG is a registered trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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Peer-Reviewed Papers
15

References

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92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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