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  • A9594 - Monoclonal ANTI-FLAG® M2-Cy3 antibody produced in mouse

A9594 Sigma-Aldrich

Monoclonal ANTI-FLAG® M2-Cy3 antibody produced in mouse

clone M2, purified immunoglobulin, buffered aqueous solution (Supplied as a solution in 10 mM sodium phosphate)

Synonym: Anti-ddddk, Anti-dykddddk, Monoclonal ANTI-FLAG® M2 antibody produced in mouse

  •  NACRES NA.32



Related Categories Alphabetical Index, Antibodies, Antibodies to Peptide and Protein Fusion Tags, FK-FO, FLAG Antibody Conjugates,
conjugate   CY3 conjugate
clone   M2, monoclonal
biological source   mouse
application(s)   direct immunofluorescence: 10 μg/mL using mammalian cells fixed with methanol:acetone
species reactivity   all
form   buffered aqueous solution (Supplied as a solution in 10 mM sodium phosphate)
shipped in   dry ice
storage temp.   −20°C
antibody form   purified immunoglobulin
isotype   IgG1
Quality Level   200
antibody product type   primary antibodies
concentration   ~1 mg/mL
immunogen sequence   DYKDDDDK


General description

Monoclonal ANTI-FLAG M2-Cy3 (mouse IgG) antibody is covalently conjugated to cyanine dye Cy3. The antibody conjugate binds to FLAG fusion proteins, and will recognize the FLAG sequence at the N-terminus, Met-N-terminus, or C-terminus of FLAG fusion proteins.


Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

For simple, one-step detection by immunocytochemistry. Especially useful in detection of FLAG fusion proteins expressed in murine host, where secondary anti-mouse antibodies might cause cross-reactivity.

Browse additional application references in our FLAG® Literature portal.

Physical form

Solution in phosphate buffered saline plus 1% BSA and preservative

Legal Information

ANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC

Cy3 is a trademark of Cytiva

FLAG is a registered trademark of Sigma-Aldrich Co. LLC


0.2, 1 mg in poly bottle

Preparation Note

Dilute the antibody in Tris buffered saline (TBS): 0.05 M Tris,pH 7.4, with 0.15 M NaCl.

Other Notes

Suggested concentration of 1-10 mg/ml for immunocytochemistry.

Safety & Documentation

Safety Information

NONH for all modes of transport
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
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Peer-Reviewed Papers


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