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B1911 Sigma-Aldrich

BCIP®/NBT Liquid Substrate System

ready to use solution

  •  NACRES NA.83




A ready-to-use, precipitating substrate system for alkaline phosphatase. This substrate system produces an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually. Immunohistochemical staining with BCIP/NBT utilizes requires organic mounting media and can be counterstained with Nuclear Fast Red or Light Green. BCIP/NBT is generally a more sensitive method than Fast Red. Not recommended for ELISA (microwell) procedures.

BCIP®/NBT Liquid Substrate System has been used:
• for staining alkaline phosphatase(ALP) in bone marrow stromal cells
• as a substrate in western blot detection of matrix metalloproteinase-2 specific antibody
• as a substrate for streptavidin-alkaline phosphatase in the enzyme-linked immunospot (ELISpot) assays


100 mL in poly bottle


It is recommended to store the product between 4 - 8 °C. Do not freeze. The product may be stored at room temperature for up to one year. If a precipitate forms, the solution can be gently swirled or filtered.

Legal Information

BCIP is a registered trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles


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Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

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Immunodetection Using BCIP/NBT Substrate

5-bromo-4-chloro-3-indolyl phosphate (BCIP)/nitro blue tetrazolium (NBT) is an ideal insoluble substrate for use with alkaline phosphatase. This system produces a blue-purple product. The intense col...
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