B4287 Sigma-Aldrich

Bovine Serum Albumin

heat shock fraction, protease free, suitable for hybridization, pH 7, ≥98%

Synonym: Albumin bovine serum, BSA, Bovine albumin



Related Categories Albumin, Albumins, Albumins by Application, Apoptosis and Cell Cycle, Biochemicals and Reagents,
Quality Level   200
biological source   bovine
grade   for molecular biology
product line   BioReagent
assay   ≥98%
form   lyophilized powder
mol wt   ~66 kDa
purified by   heat shock fractionation
packaging   poly bottle of
origin   USA origin
application(s)   ELISA: suitable
  electrophoresis: suitable
  western blot: suitable
pH   7
solubility   water: soluble (40 mg/ml)
foreign activity   Protease free
storage temp.   2-8°C
Gene Information   bovine ... ALB(280717)



Select the right albumin for your application with the Albumin Selection Guide

A non-acetylated protein suitable as a blocking agent in Southern blotting. If your application requires nuclease-free BSA, we recommend our acetylated BSA for these critical applications.


1, 5, 25 g in poly bottle

Biochem/physiol Actions

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Features and Benefits

• Protease free
• Suitable for blocking non-specific protein binding sites in hybridizations
• Heat shock fractionated

Preparation Note

Prepared using heat shock fractionation

Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

Safety & Documentation

Safety Information

Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles
Peer-Reviewed Papers


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