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CMRL-1066

powder, suitable for cell culture

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Properties

form   powder
IVD   for in vitro diagnostic use
application(s)   cell culture | mammalian: suitable
storage temp.   2-8°C

Description

General description

CMRL-1066 is a chemically defined medium developed in the late 1950’s by Connaught Medical Research Laboratories. A less complex and extensively modified version of Medium 199, CMRL-1066 was designed initially for use with L-strain cells in unsupplemented culture. It has been used to maintain monolayer growth of permanent cell lines for years without protein supplementation. Although developed for use in serum free cell culture, CMRL-1066 can be supplemented with serum and used to support the growth of many types of cells.

Application

CMRL-1066 has been used for culture of blastomeres and control embryos in vitro.

Formula variant

With L-glutamine. Without sodium bicarbonate.

Quantity

Formulated to contain 9.8 grams of powder per liter of medium.

Reconstitution

Supplement with 2.2 g/L sodium bicarbonate.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles
Peer-Reviewed Papers
15

References

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