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C1926 Sigma-Aldrich

Monoclonal Anti-Collagen, Type IV antibody produced in mouse

clone COL-94, ascites fluid

Synonym: Collagen Iv Antibody - Monoclonal Anti-Collagen, Type IV antibody produced in mouse, Collagen Iv Antibody

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Hematopoietic Stem Cells, Antibodies for Mesenchymal Stem Cells,
conjugate   unconjugated
clone   COL-94, monoclonal
biological source   mouse
application(s)   dot blot: suitable
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using protease-digested sections of human tissue
  immunohistochemistry (frozen sections): suitable
  indirect ELISA: suitable
  microarray: suitable
species reactivity   human
shipped in   dry ice
storage temp.   −20°C
antibody form   ascites fluid
isotype   IgG1
antibody product type   primary antibodies
contains   15 mM sodium azide
UniProt accession no.   P53420
Gene Information   human ... COL4A1(1282), COL4A3(1285), COL4A4(1286), COL4A5(1287), COL4A6(1288)

Description

General description

Monoclonal Anti-Collagen Type IV (mouse IgG1 isotype) is derived from the COL-94 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with human collagen Type IV.

The composition of the extracellular matrix of all vertebrates is dominated by a class of molecules known as collagens, each with a unique feature suited to its function and location. Type IV collagen is a major constituent of basement membranes. This molecule is a continuous triple helix, about 400 nm in length, composed of at least two different α chain subunits termed α1 (IV) and α2 (IV) which may be composed of the same or different molecules. Upon proteolysis, fragments of 125 kDa, 95 kDa, and 60 kDa are released from a parent α1 (IV) chain, whereas fragments of 115 kDa, 80 kDa, and 45 kDa apparently originate from a distinct α2 (IV) chain.

Specificity

Using immunohistochemical staining of basal laminae, the antibody recognizes an epitope located on the α1 and/or α2 chains of human collagen type IV. The antibody shows reactivity with native primate collagen type IV in ELISA and dot blot assay. Using the dot blot technique, the antibody shows no cross-reactivity with human collagen types I, II, III, V, VI and VII, human vitronectin, fibronectin and chondroitin sulfate types A, B and C.

Immunogen

human collagen type IV

Application

Monoclonal Anti-Collagen, Type IV antibody produced in mouse has been used in
• indirect immunolabeling
• immunofluorescence staining
• western blotting
• immunohistochemistry
• immunocytochemistry

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Collagens are known to have multiple functions. They are involved in tissue differentiation, morphogenesis and repair; play a role in the maintenance of tissue architecture; and form the filtration barrier of blood vessels.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2

Documents

Certificate of Analysis

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Milli-Q® Water Purification Solutions
Protocols & Articles

Articles

Antibody Basics

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Peer-Reviewed Papers
15

References

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