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  • C5736 - Monoclonal Anti-μ-Calpain (Calpain I, subunit p80) antibody produced in mouse

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C5736 Sigma-Aldrich

Monoclonal Anti-μ-Calpain (Calpain I, subunit p80) antibody produced in mouse

clone 15C10, purified immunoglobulin, buffered aqueous glycerol solution

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Intracellular Calcium Signaling, Antibodies to Calcium-Associated Proteins,
conjugate   unconjugated
clone   15C10, monoclonal
biological source   mouse
application(s)   immunoprecipitation (IP): 2-5 μL (native and denaturing conditions)
  indirect ELISA: suitable
  western blot (chemiluminescent): 1:1,000
species reactivity   mouse, bovine, rat, human
form   buffered aqueous glycerol solution
shipped in   wet ice
storage temp.   −20°C
antibody form   purified immunoglobulin
isotype   IgG1
antibody product type   primary antibodies
UniProt accession no.   P07384
Gene Information   human ... CAPN1(823), CAPNS1(826)
mouse ... Capn1(12333), Capns1(12336)
rat ... Capn1(29153), Capns1(29156)

Description

Specificity

Specifically recognizes the 80 kDa subunit of μ-calpain as well as two smaller proteins that are presumed to be degradation products.

Immunogen

purified human μ-calpain from placenta (calpain-I, protein kinase-C activating factor, E.C. 3.4.22.17).

Physical form

Solution in 20 mM sodium phosphate, 150 mM sodium chloride, 50% glycerol, pH 7.5, and 3 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis

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Certificate of Origin

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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Peer-Reviewed Papers
15

References

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