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  • C6164 - Cell Freezing Medium-DMSO 1×

C6164 Sigma-Aldrich

Cell Freezing Medium-DMSO 1×

sterile-filtered, suitable for cell culture

  •  NACRES NA.75



Related Categories Cell Biology, Cell Culture, Cell Freezing Media, Cell Freezing Media and Reagents, Culture,
Quality Level   100
sterility   sterile-filtered
form   liquid
application(s)   cell culture | mammalian: suitable
  cryopreservation: suitable
impurities   endotoxin, tested
shipped in   dry ice
storage temp.   −20°C


General description

Cell freezing or cryopreservation of cell culture is a routine process. It is essential to maintain the cellular integrity during storage. Majorly used cryoprotectants include glycerol, ethylene glycol and dimethyl sulfoxide(DMSO).


Cell Freezing Medium-DMSO 1× has been used in the cryopreservation of human embryonic kidney HEK293 CNR2 cells, blood mononuclear cells and prostate cancer cells.

Other Notes

Complete ready-to-use medium. Proprietary formulation with Minimum Essential Medium, dimethyl sulfoxide, calf serum and fetal bovine serum.

Safety & Documentation

Safety Information

Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
How much DMSO is present in Product C6164, Cell Freezing Medium-DMSO?
The DMSO is at a 10% concentration.
Does Product C6164, Cell Freezing Medium-DMSO, contain heat-inactivated serum?
No, the serum in this cell freezing media is not heat-inactivated.
Is there insulin in Product C6164, Cell Freezing Medium-DMSO?
No, there is no added insulin in this product.There may be traces of insulin from the serum used in this product.
Is it ok to sterile filter Product C6164, Cell Freezing Medium-DMSO?
Product No. C6164 is sterile-filtered.If the sterility of the product has been compromised, it can be re-filtered.
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Protocols & Articles


Cryopreservation of Cell Lines : Cell Culture Protocol 7

The protocol below describes the use of cell freezing methods involving an electric -80°C freezer for the cryopreservation of cell lines. ECACC routinely use a programmable rate-controlled freezer. T...
ECACC Laboratory Handbook 4th Edition
Keywords: Cryopreservation, Phase transitions

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Cell Culture Freezing Tips

Are you following best practices for cryostorage? We have cool tips for protecting your cells.
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Cell culture has become one of the most fundamental techniques for modeling biological systems, and is of increasing importance in the biotechnology and pharmaceutical sectors as well as an essential...
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