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CS0170 Sigma-Aldrich

Thioredoxin Reductase Assay Kit

 kit sufficient for 100 assays (1 mL)

  •  NACRES NA.84

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Properties

Related Categories Cell Analysis, Cell Biology, Cell Signaling and Neuroscience, Cell Stress, Nitric Oxide and Cell Stress,
Quality Level   200
usage   1 kit sufficient for 100 assays (1 mL)
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... TXNRD1(7296), TXNRD2(10587), TXNRD3(114112)

Description

General description

The Thioredoxin Reductase Assay Kit uses a colorimetric assay for the determination of thioredoxin reductase activity in mammalian cells and tissues. It is based on the reduction of 5,5′-dithiobis(2-nitrobenzoic) acid (DTNB) with NADPH to 5-thio-2-nitrobenzoic acid (TNB) that produces a strong yellow color that is measured at 412 nm.

DTNB + NADPH + H+ ↔ 2 TNB + NADP+

Application

Using post nuclear extracts of rat liver, the kit shows linearity up to 0.25 units per mL. In this preparation, ~20% of the activity is due to non-specific reductase activity (refractory to the TrxR inhibitor).

Features and Benefits

• An easy and simple method
• The kit contains all the reagents required for a colorimetric assay of mammalian thioredoxin reductase
• The kit includes an inhibitor solution for specific inhibition of mammalian thioredoxin reductase
• The kit has been tested with heart muscle, as well as lysates from cell lines such as HeLa, A549, Jurkat, U937, A431, COS, CHO, and NIH 3T3 cells

Kit component only

Description

Product #

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Assay Buffer 5x for Thioredoxin Reductase 30 mL    
Thioredoxin Reductase 10 μg    
Thioredoxin Reductase Inhibitor Solution .05 mL    
5,5'-Dithiobis(2-nitrobenzoic) acid (DNTB) 150 mg    
NADPH 25 mg    
Dimethyl Sulfoxide (DMSO) 7.5 mL    
Safety & Documentation

Safety Information

Symbol 
GHS08  GHS08
Signal word 
Warning
Hazard statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 1
Flash Point(F) 
188.6 °F
Flash Point(C) 
87 °C
Protocols & Articles
Peer-Reviewed Papers
15

References

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Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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