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CSTVRS Sigma-Aldrich

MISSION® shRNA

Custom Lentiviral Particles

To order shRNA please search for your gene of interest.


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Properties

Related Categories Functional Genomics and RNAi, MISSION shRNA, MISSION shRNA Gene Family Sets, Molecular Biology
application(s)   capture ELISA: 106 VP/mL using p24
shipped in   dry ice
storage temp.   −70°C

Description

General description

Our comprehensive shRNA product offering consists of over 150,000 pre-cloned shRNA constructs targeting more than 15,000 human and 15,000 mouse genes. The library is available in: gene family sets: gene collections related to specific functional classes such as kinases and ion channels; gene taget sets, sets of shRNA clones that target your favorite gene; and individual shRNA clones. Our shRNA and lentiviral manufacturing platforms utilize dedicated laboratories featuring state-of-the-art robotics, liquid handling and laboratory information management systems (LIMS) to provide superior quality and service for your RNAi research needs.

We provide innovative solutions for academic research, biotech research and pharmaceutical drug discovery. Our excellent systems, equipment, and personnel provide the highest quality tools that you require.

Custom Cloning - Now with TurboGFP
There may be times when classic drug selection for stable or semi-stable cell lines is not possible or desired. In addition, orthogonal methods of selection from puromycin may be required for the expression of multiple shRNAs or expression of shRNA concomitant with transgene expression.

We now offer vectors containing tGFP as a solution to these problems. This vector maintains all of the other elements of the pLKO.1 vector, but the puromycin resistance gene has been replaced by TurboGFP. Our MISSION bio-production team will customize the vector with either a TRC shRNA sequence or your favorite shRNA sequence. With TurboGFP in the vector, FACS and fluorescence microscopy techniques allow for rapid estimation of transduction efficiency and automated, rapid selection of cell populations that stably express the shRNA. Selection by GFP provides an orthogonal method when cells already stably express puromycin resistance. In addition, we also offer pLKO.1-puro-CMV-tGFP. With this vector, you can choose between GFP and puromycin selection, all with the shRNA expression vector.

For selection by a second class of antibiotics, we offer vectors containing the neomycin resistance gene. These two vectors are pLKO.1-hPGK-Neo and pLKO.1-CMV-Neo. Stable shRNA integrants can be found by selecting in G418 (Product Number A1720). As with puromycin, a cytotoxicity profile for your particular cell line will need to be generated. As an example, see the puromycin protocol, but utilize G418 instead of puromycin. As a starting point, 1 mg/mL of G418 has been utilized for selection of shRNA constructs in A549 cells.

The service includes cloning, sequencing, DNA preparation (500 ng) and lentiviral preparation (200 μl at 1 x 106 VP/mL, minimum titer via p24 assay). The DNA and viral preparations are available to be shipped 4-6 weeks from the time the order is placed. For ordering, visit our custom shRNA ordering page or contact our sales representative.

Large-Scale and High Titer Lentiviral Particles
We have a versatile manufacturing platform for high-throughput or large-scale viral production of most HIV-1-based lentiviral vectors. The standard format of the MISSION TRC shRNA collection is 200 μl of 1 x 106 VP/mL (via p24 assay) minimum titer. Any clone, target set, or control can be produced at larger volumes with the same minimum titer specification or higher titers. Our highest titer option is 109 VP/mL. We also accommodate large-scale and high titer viral preparation using clones you send to us (must be compatible with our system). Our robust and optimized processes will save you time and reduce variability in your transduction experiments. To review pricing or to submit a request for a quotation on your specific project, please use our MISSION Custom Request Submission Form.

Other Notes

For ordering any of our custom shRNA services either visit custom shRNA services, or e-mail us at MISSIONRNAi@sial.com.

To see protocols and application data please visit sigma.com/shrna.

Legal Information

Use of this product is subject to one or more license agreements.

MISSION is a registered trademark of Sigma-Aldrich Co. LLC

TurboGFP is a trademark of Evrogen Co.

Safety & Documentation

Safety Information

RIDADR 
UN3373 - class 6.2 Biological substance, Category B
WGK Germany 
WGK 2
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles

Related Content

MISSION® Lentiviral shRNA Technical Animation [VIDEO]

This video explains how MISSION RNAi (RNA interference) experiments leverage the natural RNAi mechanism. MISSION shRNA reduce the expression of specific target genes by targeting the specific mRNA th...
Keywords: Gene expression

Peer-Reviewed Papers
15

References

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SHC002H MISSION® pLKO.1-puro Non-Mammalian shRNA Control Transduction Particles, High Titer, Targets no known mammalian genes Order your shRNA target or target set in high titer format.

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SHC001V MISSION® pLKO.1-puro Empty Vector Control Transduction Particles, Contains no shRNA insert Control
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SHC005V MISSION® pLKO.1-puro eGFP shRNA Control Transduction Particles, shRNA sequence targeting eGFP Control
SHC007V MISSION® pLKO.1-puro Luciferase shRNA Control Transduction Particles, shRNA sequence targeting luciferase Control
SHM01 MISSION® ExpressMag® Super Magnetic Kit, Increases transduction efficiency The ExpressMag™ delivery system ehances your lentiviral delivery into a variety of cell types.
SHM02 MISSION® ExpressMag® 96-Well Magnetic Kit, Increases transduction efficiency The ExpressMag™ delivery system ehances your lentiviral delivery into a variety of cell types. SHM02 has been specifically designed for use with 96-well tissue culture plates.
SHC001H MISSION® pLKO.1-puro Empty Vector Control Transduction Particles, High Titer, Contains no shRNA insert High titer transduction control/negative control.
SHC004H MISSION® pLKO.1-puro TurboGFP shRNA Control Transduction Particles, High Titer, High Titer, shRNA sequence targeting tGFP High titer positive control.
SHC003H MISSION® pLKO.1-puro-CMV-TurboGFP Positive Control Transduction Particles, High Titer, TurboGFP, High Titer, Green fluorescent protein marker to monitor transduction efficiency High titer transduction control with TurboGFP.

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?