D141 Sigma-Aldrich


Used as non-ionic detergent

Synonym: Digitin

  • CAS Number 11024-24-1

  • Empirical Formula (Hill Notation) C56H92O29

  • Molecular Weight 1229.31

  •  Beilstein/REAXYS Number 78654

  •  EC Number 234-255-6

  •  MDL number MFCD00077729

  •  PubChem Substance ID 24893429

  •  NACRES NA.25



Related Categories Biochemicals and Reagents, Cell Biology, Cell Culture, Cell Lysis Reagents, Cell Signaling and Neuroscience,
Quality Level   200
optical activity   [α]20/D −54°, c = 2.8 in methanol(lit.)
mol wt   micellar avg mol wt 70,000
aggregation number   60
CMC   <0.5 mM (20-25°C)
mp   230-240 °C (dec.) (lit.)
solubility   H2O: ~5 % (w/v) (solubilized by heating to 95 °C - 98 °C and then cooling to room temp.)
SMILES string   C[C@@]12[C@]([C@@H]3C)([H])[C@](O[C@]34CC[C@@H](C)CO4)([H])[C@@H](O)[C@@]1([H])[C@@](CC[C@]5([H])[C@@]6(C[C@@H](O)[C@H](O[C@]([C@@H]([C@@H](O)[C@H]7O[C@@](O[C@H](CO)[C@@H](O)[C@@H]8O[C@@](OC[C@@H](O)[C@@H]9O)([H])[C@@H]9O)([H])[C@@H]8O[C@@](O[C@H](CO)[C@H
InChI   1S/C56H92O29/c1-19-7-10-56(75-17-19)20(2)31-45(85-56)37(67)32-22-6-5-21-11-26(24(61)12-55(21,4)23(22)8-9-54(31,32)3)76-50-42(72)39(69)44(30(16-60)80-50)81-53-48(47(36(66)29(15-59)79-53)83-49-40(70)33(63)25(62)18-74-49)84-52-43(73)46(35(65)28(14-58)78-52)82-51-41(71)38(68)34(64)27(13-57)77-51/h19-53,57-73H,5-18H2,1-4H3/t19-,20+,21+,22-,23+,24-,25-,26-,27-,28-,29-,30-,31+,32-,33+,34-,35+,36-,37+,38+,39-,40-,41-,42-,43-,44+,45-,46+,47+,48-,49+,50-,51+,52+,53+,54-,55+,56-/m1/s1



Digitonin has been used in a study to assess a procedure for isolating mitochondria from ascites tumor cells. It has also been used in a study to investigate its effect on the cytotoxicity of plant secondary metabolites in cancer cells. This mild nonionic detergent can be used to solubilize receptors and permeabilize cellular and nuclear membranes.


Preparation is free of water-insoluble constituents.

Safety & Documentation

Safety Information

Signal word 
Hazard statements 
Precautionary statements 
UN 3462 6.1 / PGIII
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
What is the solubility of Product D141, Digitonin?
The solubility of D141 is tested by heating a 5% solution in water to 95-98°C and then cooling the solution to room temperature. After cooling, the solution should remain practically clear for at least 24 hours. The product can also be dissolved at 100mg/4mL of warm ethanol.
What is the solution stability of Product D141, Digitonin?
We recommend making solutions fresh for use.
How pure is Product D141, Digitonin?
The purity of this product is evaluated by Thin Layer Chromatography. The actual results are lot specific and will be reported on the Certificate of Analysis of each lot.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
My question is not addressed here, how can I contact Technical Service for assistance?
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pHast Pack
Protocols & Articles


Solubilization of Membrane Proteins

Extracted from Purifying Challenging Proteins - Principles and Methods, GE Healthcare, 2007
Keywords: Buffers, Cell disruption, Centrifugation, Chromatography, Crystallization, Degradations, Detergents, Dot blot, Enzyme activity, Filtration, Gene expression, Indicators, Ion Exchange, Ligands, PAGE, Purification, Separation, Solvents, Western blot

Peer-Reviewed Papers


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