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D1626 Sigma

Deoxyribonucleic acid sodium salt from salmon testes

Synonym: DNA, SS-DNA, smDNA

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Description

Linkage

Formerly listed as Type III

Packaging

1, 5, 10 g in poly bottle

250 mg in poly bottle

Application

Deoxyribonucleic acid sodium salt has been used in a study to evaluate the cytotoxicity of enzyme inhibitors and absorption enhancers in Caco-2 cells for oral delivery of salmon calcitonin. Deoxyribonucleic acid sodium salt has also been used in a study to develop a simple method for extracting DNA from Cryptosporidium oocysts.
Deoxyribonucleic acid sodium salt from salmon testes has been used in basal medium for the anaerobic growth of several Bacillus mojavensis strains It has been also used as a component of immunoprecipitation (IP) buffer for the Microplate-Based Matrix Chromatin Immunoprecipitation (ChIP).

Salmon Sperm Deoxyribonucleic acid (SS-DNA) may be used to study physicochemical interactions of DNA with binding agents, intercalation agents, modification agents, detection agents and compaction agents. Salmon sperm DNA is used to study the loading capacity of magnetic mesoporous silica nanoparticles (M-MSNs) and binding capacities of polymer nanolayer architectures. SS-DNA may be used as substrate for development of DNA detection assays.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
What is the molecular weight ofProduct D1626, Deoxyribonucleic acid sodium salt from salmon testes (DNA)?
The molecular weight for product D1626 has not been determined.In general, the reference J.A.C.S., vol. 118, 10679 (1996), reports the molecular weight to be 1.3 million or around 2,000 base pairs.
What is the solubility of Product D1626, Deoxyribonucleic acid sodium salt from salmon testes (DNA)?
Sigma tests solubility in water at 2 mg/mL.To prevent shearing of the large genomic DNA, this product should be dissolved in water (1 mg/mL) with no sonication or stirring. Gentle inversion overnight at 0-4 °C is recommended to completely solubilize the DNA. The presence of 1 mM EDTA is recommended to prevent nucleases from degrading the DNA.
What is the solution stability of Product D1626, Deoxyribonucleic acid sodium salt from salmon testes (DNA)?
Solutions of DNA have been stored successfully for several months at 4°C, in 10 mM Tris, pH 7.5 - 8.0, with 1 mM EDTA and without a bacteriostatic agent.
How do I find price and availability?
There are several ways to find pricing and availability for our products.Once you log onto our website, you will find the price and availability displayed on the product detail page.You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 orview local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Protocols & Articles

Protocols

High-efficiency yeast transformation using the LiAc/SS carrier DNA/PEG method

b Department of Pathology, Environmental Health and Radiation Oncology, UCLA School of Public Health and David Geffen School of Medicine
Keywords: Antibiotics, Biochemistry, Cell biology, Cell culture, Centrifugation, Environmental, Enzymology, Evaporation, Gene expression, Genetic, Genetics, Ion-exchange chromatography, Oncology, Sterilizations, transformation

Peer-Reviewed Papers
15

References

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